The polyA tail facilitates splicing of last introns with weak 3' splice sites via PABPN1.

EMBO Rep

College of Life Sciences, TaiKang Center for Life and Medical Sciences, RNA Institute, Wuhan University, Wuhan, China.

Published: October 2023

AI Article Synopsis

  • The polyA tail of mRNAs enhances splicing of the last intron in pre-mRNAs through the action of the protein PABPN1 in HeLa cells.
  • Depletion of PABPN1 leads to retention of certain introns with weaker splice sites, indicating its critical role in splicing regulation.
  • The research highlights a new mechanism for post-transcriptional splicing regulation by showing that PABPN1 interacts with spliceosomal proteins and is important for splicing in both humans and mice.

Article Abstract

The polyA tail of mRNAs is important for many aspects of RNA metabolism. However, whether and how it regulates pre-mRNA splicing is still unknown. Here, we report that the polyA tail acts as a splicing enhancer for the last intron via the nuclear polyA binding protein PABPN1 in HeLa cells. PABPN1-depletion induces the retention of a group of introns with a weaker 3' splice site, and they show a strong 3'-end bias and mainly locate in nuclear speckles. The polyA tail is essential for PABPN1-enhanced last intron splicing and functions in a length-dependent manner. Tethering PABPN1 to nonpolyadenylated transcripts also promotes splicing, suggesting a direct role for PABPN1 in splicing regulation. Using TurboID-MS, we construct the PABPN1 interactome, including many spliceosomal and RNA-binding proteins. Specifically, PABPN1 can recruit RBM26&27 to promote splicing by interacting with the coiled-coil and RRM domain of RBM27. PABPN1-regulated terminal intron splicing is conserved in mice. Together, our study establishes a novel mode of post-transcriptional splicing regulation via the polyA tail and PABPN1.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561182PMC
http://dx.doi.org/10.15252/embr.202357128DOI Listing

Publication Analysis

Top Keywords

polya tail
20
splicing
9
intron splicing
8
splicing regulation
8
pabpn1
7
polya
6
tail facilitates
4
facilitates splicing
4
splicing introns
4
introns weak
4

Similar Publications

Keeping up with a Quickly Diversifying Pharmaceutical Landscape.

ACS Meas Sci Au

December 2024

Synthetic Molecule Analytical Chemistry, Genentech, 1 DNA Way, South San Francisco, California 94080, United States.

Small molecules and antibodies have dominated the pharmaceutical landscape for decades. However, limitations associated with therapeutic targets deemed "undruggable" and progress in biology and chemistry have led to the blossoming of drug modalities and therapeutic approaches. In 2023, a high number of 9 oligonucleotide and peptide products were approved by the Food and Drug Administration (FDA), accounting for 16% of all drugs approved.

View Article and Find Full Text PDF

Complete genome sequence of a novel iflavirus from wheat sawfly (Dolerus tritici).

Arch Virol

December 2024

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China.

Little is known about the insect viruses in wheat sawfly, Dolerus tritici, which is an important agricultural insect feeding on wheat leaves. Here, we used RNA sequencing to identify a novel single positive-strand RNA virus from the larvae of wheat sawfly collected in northern China and then determined its complete genome sequence by rapid amplification of cDNA ends. The complete genome is 9,594 nt in length, including a polyA tail at its 3' terminus, and it is predicted to encode a 326.

View Article and Find Full Text PDF

Circular RNAs in Viral Infection and Antiviral Treatment.

Cells

December 2024

Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming 650118, China.

Circular RNAs (circRNAs) are a class of noncoding RNAs that lack the 5'-cap structure and the 3' poly(A) tail. Their distinguishing feature is that the 3' and 5' ends are covalently linked to form a closed circular structure. CircRNAs have a longer half-life and stronger ribonuclease resistance compared with linear RNA.

View Article and Find Full Text PDF

Unveiling Tissue-Specific RNA Landscapes in Mouse Organs During Fasting and Feeding Using Nanopore Direct RNA Sequencing.

Adv Sci (Weinh)

December 2024

Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.

Understanding tissue-specific RNA landscapes is essential for uncovering the functional mechanisms of key organs in mammals. However, current knowledge remains limited, as short-read RNA sequencing-the predominant method for assessing gene expression-depends on incomplete gene annotations and struggles to resolve the diverse transcripts produced by genes. To address these limitations, an integrative approach combining nanopore direct RNA sequencing (DRS), ATAC-Seq, and short-read RNA-seq is used.

View Article and Find Full Text PDF

A new virus was found in Sauropus androgynus plants with curled and yellow leaves in China and tentatively named "Sauropus androgynus virus" (SaV). The complete genome of SaV is an 8007-nucleotide-long (+)RNA, excluding the 3'-poly(A) tail, and contains five open reading frames. Both pairwise comparisons and phylogenetic analysis of the putative replicase and coat proteins showed that SaV has a high level of sequence similarity to members of the genus Allexivirus of the family Alphaflexiviridae.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: