Background: Patients exquisitely sensitive to cashew/pistachio are at risk for allergic reactions to citrus seeds and pectin.
Objective: In this study, we sought to evaluate whether pectin is contaminated with citrus seeds, to identify a culprit antigen in citrus seeds, and to assess for cross-reactivity among allergens in citrus seeds, citrus pectin, and cashew or pistachio.
Methods: Proteins from orange seed coats, orange seed endosperms, lemon seeds, grapefruit seeds, citrus pectin, apple pectin, and grapefruit pectin were extracted. Protein concentrations in all extracts were determined and visualized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique. Immunoglobulin E-binding capacity was determined with Western blot analyses and tandem mass spectrometry for the identification of the culprit allergen in citrus seeds and pectin.
Results: In subjects with citrus seed, pectin, and cashew allergies, there was strong immunoglobulin E-reactivity to bands between 17 to 28 kDa and 28 to 38 kDa. The tandem mass spectrometry analysis of these bands indicated the presence of citrin as the culprit allergen. Citrin and Ana o 2 are both 11S globulins belonging to the cupin superfamily, and significant homology was found between these proteins.
Conclusion: Citrus pectin may be contaminated with citrus seeds. Citrin, a newly identified allergen in citrus seeds, seems to be the culprit antigen in citrus seeds and contaminated citrus pectin. Citrin is highly homologous with Ana o 2 in cashew and Pis v 2 in pistachio, suggesting potential for cross-reactivity and providing an explanation for co-allergenicity of cashew or pistachio, citrus seeds, and citrus pectin.
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http://dx.doi.org/10.1016/j.anai.2023.08.603 | DOI Listing |
Plants (Basel)
December 2024
Department of Life Science, Health, and Health Professions, University of Rome "Link Campus", 00165 Rome, Italy.
Mandarin, one of the winter fruits commonly used in the preparation of foods and juices, is a fruit native to China and Southeast Asia. In this work, essential oils (EOs) obtained from by-products of the Blanco flavedo of five cultivars present and cultivated within the Botanical Garden of Palermo were chemically and biologically studied: 'Avana' (), 'Tardivo di Ciaculli' (), 'Bombajensis' (), 'Aurantifolia' (), and 'Padre Bernardino' (). The GC and GC-MS analysis performed on all the extracted samples clearly highlighted the notable presence of limonene, a characteristic hydrocarbon monoterpene of EOs of the genus.
View Article and Find Full Text PDFBackground: Diaphorina citri is an insect vector of "Candidatus Liberibacter asiaticus" (CLas), the gram-negative bacterial pathogen associated with citrus greening disease. Control measures rely on pesticides with negative impacts on the environment, natural ecosystems, and human and animal health. In contrast, gene-targeting methods have the potential to specifically target the vector species and/or reduce pathogen transmission.
View Article and Find Full Text PDFFoods
November 2024
School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China.
Using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technology in multiple reaction monitoring mode, a widely targeted metabolomics approach was employed to identify metabolites in five tissues (exocarp, endocarp, segment membrane, pulp, and seeds) of the Shatian pomelo fruit. The differences in metabolite composition and abundance among different tissues were analyzed using multivariate statistical analysis methods. The results showed that a total of 1722 metabolites were identified from the five tissues of the Shatian pomelo, including 413 flavonoids and 277 amino acids and their derivatives.
View Article and Find Full Text PDFArerugi
November 2024
Department of Pediatrics, Jichi Medical University Saitama Medical Center.
The case is a 12-year-old girl. She was diagnosed with cashew nut allergy in infancy. She experienced the following allergic symptoms at age 11: sore throat after ingesting gummy bears containing citrus-derived pectin; sore throat, nausea, and severe abdominal pain after ingesting jelly containing yuzu (citrus junos) seeds and peel.
View Article and Find Full Text PDFHeliyon
November 2024
Horticultural Department, Faculty of Agriculture, Suez Canal University, 41522, Egypt.
Phosphorus (P) is considered as an essential element in crop production. Besides, the study of P from the elemental and bio sources impacts on productivity and quality of orange trees could emphasize its utilization importance. This study aimed to evaluate the chemical implication of three treatments of P fertilization (Triple phosphate (TP; 620 and 900 g/tree/year), phosphate rock (PR; 1820 and 2742 g/tree/year) and 1:1 TP:PR on Valencia orange trees ( L.
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