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Imaging flow cytometry combines the high throughput nature of flow cytometry with the advantages of single cell image acquisition associated with microscopy. The measurement of large numbers of features from the resulting images provides rich datasets which have resulted in a wide range of novel biomedical applications. In this primer we discuss the typical imaging flow instrumentation, the form of data acquired and the typical analysis tools that can be applied to this data. Using examples from the literature we discuss the progression of the analysis methods that have been applied to imaging flow cytometry data. These methods start from the use of simple single image features and multiple channel gating strategies, followed by the design and use of custom features for phenotype classification, through to powerful machine and deep learning methods. For each of these methods, we outline the processes involved in analyzing typical datasets and provide details of example applications. Finally we discuss the current limitations of imaging flow cytometry and the innovations which are addressing these challenges.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468826 | PMC |
http://dx.doi.org/10.1038/s43586-022-00167-x | DOI Listing |
Ann Ital Chir
March 2025
Department of Ultrasound, The First Affiliated Hospital of the Air Force Medical University, 710032 Xi'an, Shaanxi, China.
Aims: This study combined a new ultrasound venous filling degree (VFD) parameter with the Caprini score to assess the clinical value of the Caprini score in predicting deep venous thrombosis (DVT) of the lower extremities.
Methods: This retrospective study included 150 inpatients undergoing orthopedic lower extremity surgery at the First Affiliated Hospital of the Air Force Medical University between June 2023 and June 2024. They included 41 (27.
Vet Anaesth Analg
February 2025
Langford Vets, University of Bristol, Langford, UK.
An 8-year-old male neutered British Shorthair cat, weighing 5.12 kg, was referred for chronic mild inappetence, lethargy, bilirubinuria, weight loss and icterus. Diagnostic imaging and serum biochemistry suggested a partial biliary tract obstruction caused by an enlarged pancreas.
View Article and Find Full Text PDFPhotodiagnosis Photodyn Ther
March 2025
Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China; Present address: Department of Physiology, University of California, San Francisco, CA, 94107, USA. Electronic address:
Background: While dental pulp stem cells (DPSCs) show therapeutic potential for spinal cord injury (SCI), post-traumatic oxidative stress and mitochondrial dysfunction critically compromise grafted cell survival. Photobiomodulation therapy (PBMT) is a noninvasive approach that may enhance regenerative efficacy. This study investigates whether PBMT improves the survival and neural differentiation of transplanted DPSCs in SCI models.
View Article and Find Full Text PDFJ Biomech
March 2025
Department of Mechanical Engineering, University College London, London, UK; UCL Hawkes Institute, University College London, London, UK. Electronic address:
Peripheral Arteriovenous Malformations (pAVMs) are congenital vascular anomalies characterised by abnormal connections between arteries and veins that bypass the capillary network. This bypass results on a high-flow and low resistance vascular structure termed nidus. The high-flow and complex angioarchitecture of pAVMs makes treatment challenging and often suboptimal, as evidenced by high recurrence rates.
View Article and Find Full Text PDFJ Neurol Sci
March 2025
Department of Radiology & Nuclear Medicine, Maastricht University Medical Center, the Netherlands; Mental Health & Neuroscience Research Institute (MHeNs), Maastricht University, the Netherlands; Department of Electrical Engineering, Eindhoven University of Technology, the Netherlands. Electronic address:
Objective: Coronavirus disease 2019 (COVID-19) is known to have adverse effects on the brain's vasculature in some patients. After recovery of the infection, vascular abnormalities may persist, but it remains unclear which pathological pathways play a role in post-COVID vascular and cognitive dysfunction and may contribute to post-COVID cognitive complaints.
Methods: In this observational cohort study, 108 previously hospitalized COVID-19 survivors (general ward: n = 53; intensive care unit (ICU): n = 55) were compared.
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