As an important emerging pollutant, antibiotic resistance genes (ARGs) monitoring is crucial to protect the ecological environment and public health, but its rapid and accurate detection is still a major challenge. In this study, a new single-labeled dual-signal output ratiometric electrochemical genosensor (E-DNA) was developed for the rapid and highly sensitive detection of ARGs using a synergistic signal amplification strategy of TC@Au nanoparticles (TC@AuNPs) and isothermal strand displacement polymerase reaction (ISDPR). Specially, two-dimensional monolayer TC nanosheets loaded with uniformly gold nanoparticles were prepared and used as the sensing platform of the E-DNA sensor. Benefiting from excellent conductivity and large specific surface area of TiC@AuNPs, the probe immobilization capacity of the E-DNA sensor is doubled, and electrochemical response signals of the E-DNA sensor were significantly improved. The proposed single-labeled dual-signal output ratiometric sensing strategy exhibits three to six times higher sensitivity for the sul2 gene than the single-signal sensing strategy, which significantly reduces cost meanwhile retaining the advantages of high sensitivity and reliability offered by conventional dual-labeled ratiometric sensors. Coupled with ISDPR amplification technology, the E-DNA sensor has a wider linear range from 10 fM to 10 nM and a limit of detection as low as 2.04 fM (S/N=3). More importantly, the E-DNA sensor demonstrates excellent specificity, good stability and reproducibility for target ARGs detection in real water samples. The proposed new sensing strategy provides a highly sensitive and versatile tool for the rapid and accurate quantitative analysis of various ARGs in environmental water samples.
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http://dx.doi.org/10.1016/j.bios.2023.115643 | DOI Listing |
Anal Methods
November 2024
School of Materials Science and Engineering, Jiangxi Provincial Key Laboratory of Power Batteries & Energy Storage Materials, Jiangxi University of Science and Technology, Ganzhou 341000, China.
The present work demonstrates a label-free, rapid and ultrasensitive electrochemical sensor for specific DNA detection with an exonuclease III (Exo III)-assisted target recycling amplification strategy and elevated electrode temperature at a heated gold disk electrode (HAuDE). The proposed electrochemical DNA (E-DNA) sensor was designed such that in the presence of the target DNA, the electrode self-assembled capture probe hybridizes with the target DNA to form a duplex structure, which triggers Exo III to specifically recognize this structure and selectively digest the capture probe, while the released target DNA underwent recycling to hybridize with a new capture probe, leading to the gradual digestion of a large amount of capture probes. It was found that during the digestion period, the activity of Exo III could be significantly improved by elevating electrode temperature, thus promoting the digestion reaction and improving the sensitivity for target DNA detection.
View Article and Find Full Text PDFBiomimetics (Basel)
December 2023
Advanced Manufacturing Engineering Laboratory, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-0055, Japan.
Smart manufacturing needs cognitive computing methods to make the relevant systems more intelligent and autonomous. In this respect, bio-inspired cognitive computing methods (i.e.
View Article and Find Full Text PDFTrends Ecol Evol
March 2024
Kunming Institute of Zoology; Yunnan, China; University of East Anglia, Norfolk, UK.
Bioelectrochemistry
February 2024
Chemistry of Surfaces, Interfaces and Nanomaterials (ChemSIN), Faculté des Sciences, Université libre de Bruxelles (ULB), Boulevard du Triomphe, 2, CP 255, B-1050 Bruxelles, Belgium. Electronic address:
The electrochemical properties of three oxazine fluorophores, ATTO655, ATTO680 and ATTO700 have been investigated at gold electrodes. They display a reversible or quasi-reversible voltammetric behaviour involving either a 2e, 2H or a 2e, 1H redox process depending on the pH, at a formal potential located in the stability range of thiolate self-assembled monolayers (E°' ≈ -0.33 V vs.
View Article and Find Full Text PDFBiosens Bioelectron
November 2023
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China. Electronic address:
As an important emerging pollutant, antibiotic resistance genes (ARGs) monitoring is crucial to protect the ecological environment and public health, but its rapid and accurate detection is still a major challenge. In this study, a new single-labeled dual-signal output ratiometric electrochemical genosensor (E-DNA) was developed for the rapid and highly sensitive detection of ARGs using a synergistic signal amplification strategy of TC@Au nanoparticles (TC@AuNPs) and isothermal strand displacement polymerase reaction (ISDPR). Specially, two-dimensional monolayer TC nanosheets loaded with uniformly gold nanoparticles were prepared and used as the sensing platform of the E-DNA sensor.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!