AI Article Synopsis
- T-cell receptor (TR) diversity and structure are critical for understanding immune responses in vertebrates, but studying these in non-model organisms has been challenging due to poor genome assemblies.
- High-quality genome assemblies for salmonids, specifically rainbow trout, have now made it possible to analyze their TRB loci, located on chromosomes 19 and 25.
- This research reveals that salmonids have a consistent TRB locus structure, contributing to our understanding of their evolutionary history and providing a foundation for future studies on T cell responses through TRB repertoire sequencing.
Article Abstract
T-cell mediated immunity relies on a vast array of antigen specific T cell receptors (TR). Characterizing the structure of TR loci is essential to study the diversity and composition of T cell responses in vertebrate species. The lack of good-quality genome assemblies, and the difficulty to perform a reliably mapping of multiple highly similar TR sequences, have hindered the study of these loci in non-model organisms. High-quality genome assemblies are now available for the two main genera of Salmonids, and . We present here a full description and annotation of the TRB loci located on chromosomes 19 and 25 of rainbow trout (). To get insight about variations of the structure and composition of TRB locus across salmonids, we compared rainbow trout TRB loci with other salmonid species and confirmed that the basic structure of salmonid TRB locus is a double set of two TRBV-D-J-C loci in opposite orientation on two different chromosomes. Our data shed light on the evolution of TRB loci in Salmonids after their whole genome duplication (WGD). We established a coherent nomenclature of salmonid TRB loci based on comprehensive annotation. Our work provides a fundamental basis for monitoring salmonid T cell responses by TRB repertoire sequencing.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464911 | PMC |
| http://dx.doi.org/10.3389/fimmu.2023.1238321 | DOI Listing |
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