AI Article Synopsis
- Dihydrochalcones (DHCs), like phlorizin and trilobatin, are key phenylpropanoids found in apples, playing significant roles in plant-insect interactions and human health.
- The synthesis of these compounds is controlled by specific genes, and studies revealed that their production is determined by the activities of the promoters of two glycosyltransferase genes, PGT1 and PGT2.
- Transcription factors with MYB-like domains regulate the accumulation of DHCs in various apple tissues, highlighting their essential role in seed development and explaining the absence of apple varieties lacking DHCs.
Article Abstract
Dihydrochalcones (DHCs) including phlorizin (phloretin 2'-O-glucoside) and its positional isomer trilobatin (phloretin 4'-O-glucoside) are the most abundant phenylpropanoids in apple (Malus spp.). Transcriptional regulation of DHC production is poorly understood despite their importance in insect- and pathogen-plant interactions in human physiology research and in pharmaceuticals. In this study, segregation in hybrid populations and bulked segregant analysis showed that the synthesis of phlorizin and trilobatin in Malus leaves are both single-gene-controlled traits. Promoter sequences of PGT1 and PGT2, two glycosyltransferase genes involved in DHC glycoside synthesis, were shown to discriminate Malus with different DHC glycoside patterns. Differential PGT1 and PGT2 promoter activities determined DHC glycoside accumulation patterns between genotypes. Two transcription factors containing MYB-like DNA-binding domains were then shown to control DHC glycoside patterns in different tissues, with PRR2L mainly expressed in leaf, fruit, flower, stem, and seed while MYB8L mainly expressed in stem and root. Further hybridizations between specific genotypes demonstrated an absolute requirement for DHC glycoside production in Malus during seed development which explains why no Malus spp. with a null DHC chemotype have been reported.
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| http://dx.doi.org/10.1111/tpj.16444 | DOI Listing |
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Article Synopsis
- Dihydrochalcones (DHCs), like phlorizin and trilobatin, are key phenylpropanoids found in apples, playing significant roles in plant-insect interactions and human health.
- The synthesis of these compounds is controlled by specific genes, and studies revealed that their production is determined by the activities of the promoters of two glycosyltransferase genes, PGT1 and PGT2.
- Transcription factors with MYB-like domains regulate the accumulation of DHCs in various apple tissues, highlighting their essential role in seed development and explaining the absence of apple varieties lacking DHCs.
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