AI Article Synopsis
- Liver diseases are a leading cause of health issues globally, and 3D bioprinting offers a solution for overcoming challenges like organ shortages and immune rejection by creating functional living tissues, including liver tissues.
- 3D bioprinting techniques, such as inkjet and extrusion-based methods, utilize bioinks made of hydrogels and specific liver cells to accurately replicate the complex structures of native tissues, improving the potential for disease modeling and regenerative medicine.
- Despite its advantages, 3D bioprinting faces challenges, including the need for advanced fabrication technologies and difficulties in replicating the liver's microenvironment, yet it remains a promising area of innovation in biofabrication.
Article Abstract
Liver diseases are the primary reason for morbidity and mortality in the world. Owing to a shortage of organ donors and postoperative immune rejection, patients routinely suffer from liver failure. Unlike 2D cell models, animal models, and organoids, 3D bioprinting can be successfully employed to print living tissues and organs that contain blood vessels, bone, and kidney, heart, and liver tissues and so on. 3D bioprinting is mainly classified into four types: inkjet 3D bioprinting, extrusion-based 3D bioprinting, laser-assisted bioprinting (LAB), and vat photopolymerization. Bioinks for 3D bioprinting are composed of hydrogels and cells. For liver 3D bioprinting, hepatic parenchymal cells (hepatocytes) and liver nonparenchymal cells (hepatic stellate cells, hepatic sinusoidal endothelial cells, and Kupffer cells) are commonly used. Compared to conventional scaffold-based approaches, marked by limited functionality and complexity, 3D bioprinting can achieve accurate cell settlement, a high resolution, and more efficient usage of biomaterials, better mimicking the complex microstructures of native tissues. This method will make contributions to disease modeling, drug discovery, and even regenerative medicine. However, the limitations and challenges of this method cannot be ignored. Limitation include the requirement of diverse fabrication technologies, observation of drug dynamic response under perfusion culture, the resolution to reproduce complex hepatic microenvironment, and so on. Despite this, 3D bioprinting is still a promising and innovative biofabrication strategy for the creation of artificial multi-cellular tissues/organs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457767 | PMC |
| http://dx.doi.org/10.3390/mi14081648 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions.
Trends Biotechnol
January 2025
State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, 310058, People's Republic of China; School of Mechanical Engineering, Zhejiang University, Hangzhou, 310058, People's Republic of China. Electronic address:
Replicating the contractile function of arterial tissues in vitro requires precise control of cell alignment within 3D structures, a challenge that existing bioprinting techniques struggle to meet. In this study, we introduce the voxel-based embedded construction for tailored orientational replication (VECTOR) method, a voxel-based approach that controls cellular orientation and collective behavior within bioprinted filaments. By fine-tuning voxel vector magnitude and using an omnidirectional printing trajectory, we achieve structural mimicry at both the macroscale and the cellular alignment level.
View Article and Find Full Text PDFAdvancing plant science through precision 3D bioprinting: new tools for research and biotech applications.
Curr Opin Biotechnol
January 2025
Department of Plant and Microbial Biology and NC Plant Sciences Initiative, North Carolina State University, Raleigh, NC 27695, USA. Electronic address:
The integration of 3D bioprinting into plant science and biotechnology is revolutionizing research and applications. While many high-throughput techniques have advanced plant biology, replicating the complex 3D organization and cellular environments of plant tissues remains a significant challenge. Traditional 2D culture systems fall short of capturing the necessary spatial context for accurate studies of cell behavior, gene expression, and tissue development.
View Article and Find Full Text PDFEffects of Magnesium-Doped Hydroxyapatite Nanoparticles on Bioink Formulation for Bone Tissue Engineering.
ACS Appl Bio Mater
January 2025
Center of Translational Oral Research (TOR), Department of Clinical Dentistry, University of Bergen, 5009 Bergen, Norway.
Bioprinting of nanohydroxyapatite (nHA)-based bioinks has attracted considerable interest in bone tissue engineering. However, the role and relevance of the physicochemical properties of nHA incorporated in a bioink, particularly in terms of its printability and the biological behavior of bioprinted cells, remain largely unexplored. In this study, two bioinspired nHAs with different chemical compositions, crystallinity, and morphologies were synthesized and characterized: a more crystalline, needle-like Mg-doped nHA (N-HA) and a more amorphous, rounded Mg- and CO-doped nHA (R-HA).
View Article and Find Full Text PDFDecellularization of fish tissues for tissue engineering and regenerative medicine applications.
Regen Biomater
November 2024
Zhejiang Top-Medical Medical Dressing Co. Ltd, Wenzhou, Zhejiang 325025, China.
Decellularization is the process of obtaining acellular tissues with low immunogenic cellular components from animals or plants while maximizing the retention of the native extracellular matrix structure, mechanical integrity and bioactivity. The decellularized tissue obtained through the tissue decellularization technique retains the structure and bioactive components of its native tissue; it not only exhibits comparatively strong mechanical properties, low immunogenicity and good biocompatibility but also stimulates neovascularization at the implantation site and regulates the polarization process of recruited macrophages, thereby promoting the regeneration of damaged tissue. Consequently, many commercial products have been developed as promising therapeutic strategies for the treatment of different tissue defects and lesions, such as wounds, dura, bone and cartilage defects, nerve injuries, myocardial infarction, urethral strictures, corneal blindness and other orthopedic applications.
View Article and Find Full Text PDF3D Printing in Wound Healing: Innovations, Applications, and Future Directions.
Cureus
December 2024
Department of Pharmaceutics, Krishna Institute of Pharmacy, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad, IND.
The field of wound healing faces significant challenges, particularly in the treatment of chronic wounds, which often result in prolonged healing times and complications. Recent advancements in 3D printing technology have provided innovative solutions to these challenges, offering tailored and precise approaches to wound care. This review highlights the role of 3D printing in enhancing wound healing, focusing on its application in creating biocompatible scaffolds, custom wound dressings, and drug delivery systems.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!