Transcription factors containing a CCCH structure (C3H) play important roles in plant growth and development, and their stress response, but research on the gene family in potato has not been reported yet. In this study, we used bioinformatics to identify 50 genes in potato and named them to according to their location on chromosomes, and we analyzed their physical and chemical properties, chromosome location, phylogenetic relationship, gene structure, collinearity relationship, and cis-regulatory element. The gene expression pattern analysis showed that many genes are involved in potato growth and development, and their response to diverse environmental stresses. Furthermore, RT-qPCR data showed that the expression of many genes was induced by high temperatures, indicating that genes may play important roles in potato response to heat stress. In addition, Some genes were predominantly expressed in the stolon and developing tubers, suggesting that these genes may be involved in the regulation of tuber development. Together, these results provide new information on genes and will be helpful for further revealing the function of genes in the heat stress response and tuber development in potato.
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http://dx.doi.org/10.3390/ijms241612888 | DOI Listing |
Biology (Basel)
December 2024
Wulanchabu Academy of Agricultural and Forestry Sciences, Wulanchabu 012000, China.
The Solanaceae family, which includes vital crops such as tomatoes, peppers, eggplants, and potatoes, is increasingly impacted by drought due to climate change. Recent research has concentrated on unraveling the molecular mechanisms behind drought resistance in these crops, with a focus on abscisic acid (ABA) signaling pathways, transcription factors (TFs) like MYB (Myeloblastosis), WRKY (WRKY DNA-binding protein), and NAC (NAM, ATAF1/2, and CUC2- NAM: No Apical Meristem, ATAF1/2, and CUC2: Cup-shaped Cotyledon), and the omics approaches. Moreover, transcriptome sequencing (RNA-seq) has been instrumental in identifying differentially expressed genes (DEGs) crucial for drought adaptation.
View Article and Find Full Text PDFPhytopathology
January 2025
Swedish University of Agricultural Sciences, Plant Protection Biology, Alnarp, Sweden;
Transglutaminases (TGases) are enzymes highly conserved among prokaryotic and eukaryotic organisms, where their role is to catalyze protein cross-linking. One of the putative TGases of has previously been shown to be localized to the cell wall. Based on sequence similarity we were able to identify six more genes annotated as putative TGases and show that these seven genes group together in phylogenetic analysis.
View Article and Find Full Text PDFProtein Expr Purif
December 2024
Gujarat Biotechnology Research Centre, Gandhinagar, 382011, Gujarat, India. Electronic address:
Plant glucanases, including potato glucanase, are pivotal in biological processes such as cell growth, development, and defense against pathogens. These enzymes hold substantial promises in biotechnological applications, especially genetic engineering for enhancing crop disease resistance and stress tolerance. In this study, from Solanum tuberosum, glycosyl hydrolases family 17 (GH-17) β-1,3-glucanase (Stglu) was cloned, expressed, characterized and its antifungal activity was evaluated.
View Article and Find Full Text PDFUltrason Sonochem
January 2025
Department of Family and Consumer Sciences, North Carolina A&T State University, Greensboro, NC 27411, USA. Electronic address:
Sweet potatoes are a rich source of nutrients and bioactive compounds, but their quality can be impacted by the drying process. This study investigates the impact of slot jet reattachment (SJR) nozzle and ultrasound (US) combined drying (SJR + US) on sweet potato quality, compared to freeze-drying (FD), SJR drying, and hot air drying (HAD). SJR + US drying at 50 °C closely resembled FD in enhancing quality attributes and outperformed HAD and SJR in key areas such as rehydration, shrinkage ratios, and nutritional composition.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China.
Late Embryogenesis Abundant (LEA) proteins are extensively distributed among higher plants and are crucial for regulating growth, development, and abiotic stress resistance. However, comprehensive data regarding the LEA gene family in Ipomoea species remains limited. In this study, we conducted a genome-wide comparative analysis across seven Ipomoea species, including sweet potato (I.
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