A prompt on-site, real-time method to detect bacterial antibiotic resistance is crucial for controlling the spread of resistance. Herein, we report the use of surface-enhanced Raman spectroscopy (SERS) for the monitoring of bioactive metabolites produced by ampicillin-resistant strains and identification of mechanisms underlying antibiotic resistance. The results indicate that the blue-green pigment pyocyanin (PYO) dominates the metabolite signals and is significantly enhanced upon exposure to subminimal inhibitory concentrations of ampicillin. PYO accumulates during exponential growth and subsequently either diffuses into the culture medium or is consumed in response to nutrient deprivation. The SERS spectra further reveal that the production of some intermediate substances such as polysaccharides and amino acids is minimally impacted and that nutrient consumption remains consistent. Moreover, the intensity changes and peak shifts observed in the SERS spectra of non-PYO-producing ampicillin-susceptible demonstrate that exogenously added PYO enhances tolerance to ampicillin to some extent. These results indicate that PYO mediates antibiotic resistance not only in the parent species but also in cocultured bacterial strains. The metabolic SERS signal provides new insight regarding antibiotic resistance with promising applications for both environmental monitoring and rapid clinical detection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.est.3c04001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Onabotulinum Toxin A-Led Urinary Tract Infections-Should we Safeguard? A Randomized Controlled Trial.
Int Urogynecol J
January 2025
Department of Urogynecology and Reconstructive Pelvic Surgery, Atlantic Health System, 435 South Street, Suite 370, Morristown, NJ, 07960, USA.
Introduction And Hypothesis: The objective of our study was to evaluate the need for antibiotic prophylaxis for urinary tract infection (UTI) prevention before Onabotulinum toxin A injection for overactive bladder (OAB). We hypothesize that the lack of antibiotic prophylaxis might not be inferior to administering prophylaxis.
Methods: This was a multi-centered, nonblinded, randomized controlled trial conducted between August 2022 and September 2024.
Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway.
Eur J Histochem
January 2025
Department of Critical Care Medicine, The Qujing No.1 People's Hospital, Qujing.
Intestinal barrier damage causes an imbalance in the intestinal flora and microbial environment, promoting a variety of gastrointestinal diseases. This study aimed to explore the mechanism by which adipose-derived stem cells (ADSCs) repair intestinal barrier damage. The human colon adenocarcinoma cell line Caco-2 and rats were treated with lipopolysaccharide (LPS) to establish in vitro and in vivo models, respectively, of intestinal barrier damage.
View Article and Find Full Text PDFBacterium-Phage Symbiosis Facilitates the Enrichment of Bacterial Pathogens and Antibiotic-Resistant Bacteria in the Plastisphere.
Environ Sci Technol
January 2025
College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
The plastisphere, defined as the ecological niche for microbial colonization of plastic debris, has been recognized as a hotspot of pathogenic and antibiotic-resistant bacteria. However, the interactions between bacteria and phages facilitated by the plastisphere, as well as their impact on microbial risks to public health, remain unclear. Here, we analyzed public metagenomic data from 180 plastisphere and environmental samples, stemming from four different habitats and two plastic types (biodegradable and nonbiodegradable plastics) and obtained 611 nonredundant metagenome-assembled genomes (MAGs) and 4061 nonredundant phage contigs.
View Article and Find Full Text PDFMolecular Characterization of Whole Genome Sequencing of spp. in Shapingba District, Chongqing, China, 2016-2023.
Foodborne Pathog Dis
January 2025
Center for Disease Control and Prevention of Sichuan Province, Chengdu, China.
In recent years, infection is a major global public health concern, particularly in food safety. This study analyzed the genomes of 102 strains isolated between 2016 and 2023 from food, foodborne disease patients, and food poisoning incidents, focusing on their molecular characteristics, antibiotic resistance genes (ARGs), and virulence genes. serovar Enteritidis (37.
View Article and Find Full Text PDFSafety and Tolerability of Pivmecillinam During More Than Four Decades of Clinical Experience: A Systematic Review.
Clin Infect Dis
January 2025
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
The recent US Food and Drug Administration approval of pivmecillinam-an oral prodrug of the amidinopenicillin antibiotic mecillinam-presents a valuable opportunity to address the need for new treatments for uncomplicated urinary tract infection (uUTI). We report findings of a systematic literature review of the safety profile of pivmecillinam/mecillinam based on more than 40 years' experience, mainly in Europe and Canada, to describe its tolerability profile and identify any important safety signals. In total, 110 eligible publications were identified describing use of pivmecillinam/mecillinam as monotherapy or in combination, for treatment of uUTI or other infectious conditions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!