AI Article Synopsis
- The study investigates how the processing stages of a medicinal orchid species (referred to as "Fengdou") affect its metabolites, using techniques like metabolomics and network pharmacology.
- A total of 628 metabolites were detected, with 109 identified as differential metabolites that display potential pharmacological activity.
- The findings suggest that the processing enhances the levels of certain beneficial metabolites, which may contribute to anticoagulant, hypoglycemic, and tumor-inhibiting effects, thus improving the orchid's medicinal quality.
Article Abstract
() is a precious medicinal species of Orchidaceae, and the product obtained by hot processing is called "Fengdou". At present, the research on the processing quality of mainly focuses on the chemical composition indicators such as polysaccharides and flavonoids content. However, the changes in metabolites during processing are still unclear. In this study, the process was divided into two stages and three important conditions including fresh stems, semiproducts and "Fengdou" products. To investigate the effect of processing on metabolites of in different processing stages, an approach of combining metabolomics with network pharmacology and molecular docking was employed. Through UPLC-MS/MS analysis, a total of 628 metabolites were detected, and 109 of them were identified as differential metabolites (VIP ≥ 1, |log2 (FC)| ≥ 1). Next, the differential metabolites were analyzed using the network pharmacology method, resulting in the selection of 29 differential metabolites as they have a potential pharmacological activity. Combining seven diseases, 14 key metabolites and nine important targets were screened by constructing a metabolite-target-disease network. The results showed that seven metabolites with potential anticoagulant, hypoglycemic and tumor-inhibiting activities increased in relative abundance in the "Fengdou" product. Molecular docking results indicated that seven metabolites may act on five important targets. In general, processing can increase the content of some active metabolites of and improve its medicinal quality to a certain extent.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456568 | PMC |
| http://dx.doi.org/10.3390/metabo13080886 | DOI Listing |
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