Background: The treatment of paracoccidioidomycosis (PCM) is a challenge, and the discovery of new antifungal compounds is crucial. The phenacylideneoxindoles exhibited promising antifungal activity against spp., but their mode of action remains unknown.
Methods: Through proteomic analysis, we investigated the effects of (E)-3-(2-oxo-2-phenylethylidene)indolin-2-one on . In addition, we investigated the metabolic alterations of in response to the compound. Furthermore, the effects of the compound on the membrane, ethanol production, and reactive oxygen species (ROS) production were verified.
Results: We identified differentially regulated proteins that revealed significant metabolic reorganization, including an increase in ethanol production, suggesting the activation of alcoholic fermentation and alterations in the rigidity of fungal cell membrane with an increase of the ergosterol content and formation of ROS.
Conclusions: These findings enhance our understanding of the mode of action and response of to the investigated promising antifungal compound, emphasizing its potential as a candidate for the treatment of PCM.
Laboratory of Spectroscopy, Molecular Modelling, Materials, Nanomaterial, Water and Environment, CERNE2D, Mohammed V University in Rabat, Faculty of Science, Rabat, Morocco.
Introduction: Morocco is home to a remarkable diversity of flora, including several species from the Artemisia genus. This study aims to thoroughly examine the chemical composition of essential oils derived from Artemisia species and assess their antibacterial and antioxidant properties through in vitro experiments and in silico simulations.
Aim: Voriconazole (VRZ) is highly effective in treating invasive pulmonary aspergillosis (IPA), in addition to hepatotoxicity. Therefore, the current study focuses on the development and characterization of voriconazole-loaded microspheres (VRZ@PCL MSPs) to augment pulmonary localization and antifungal efficacy.
Methods: VRZ@PCL MSPs were fabricated by using the o/w emulsion method.
Background: Acute myeloid leukemia (AML) is a heterogeneous hematological malignancy caused by disorders in stem cell differentiation and excessive proliferation resulting in clonal expansion of dysfunctional cells called myeloid blasts. The combination of chemotherapeutic agents with natural product-based molecules is promising in the treatment of AML. In this study, we aim to investigate the anti-cancer effect of Rapamycin and Niacin combination on THP-1 and NB4 AML cell lines.
The study examines the chemical composition and antimicrobial properties of petroleum ether and hydro-methanolic extracts of Achillea santolina(A. santolina) from Algeria. Chemical profiling was performed using High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) for the hydro-methanolic extract and Gas Chromatography-Mass Spectrometry (GC-MS) for the petroleum ether extract.
Objectives: To evaluate the antifungal activity of extracts and compounds from against clinically relevant species, notably , and investigate possible mechanisms of action using electron microscopy and techniques.
Methods: Extracts and fractions of were obtained through turboextraction and partitioning, while the isolated compounds were previously purified. The ethyl acetate fraction (EAF) was characterized by HPLC.
