MicroRNA miR-274-5p Suppresses Associated with Melanotic Mass Formation and Developmental Growth in .

The hematopoietic system plays a crucial role in immune defense response and normal development, and it is regulated by various factors from other tissues. The dysregulation of hematopoiesis is associated with melanotic mass formation; however, the molecular mechanisms underlying this process are poorly understood. Here, we observed that the overexpression of in the fat body resulted in the formation of melanotic masses. Moreover, abnormal activation of the JNK and JAK/STAT signaling pathways was linked to these consequences. In addition to this defect, overexpression in the larval fat body decreased the total tissue size, leading to a reduction in body weight. miR-274-5p was found to directly suppress the expression of (), which encodes an RNA-binding protein. Similar to the effects of overexpression, depletion led to melanotic mass formation and growth reduction. Collectively, miR-274 plays a regulatory role in the -JNK signaling axis in melanotic mass formation and growth control.