Mitophagy is a process whereby cells selectively remove mitochondria through the mechanism of autophagy, which plays an important role in maintaining cellular homeostasis. In order to explore the effect of mitophagy genes on the antioxidant activities of , mutants with deletion or overexpression of mitophagy genes , and were constructed respectively. The results indicated that overexpression of and genes significantly reduced the intracellular reactive oxygen species (ROS) content upon HO stress for 6 h, which were 61.23% and 46.35% of the initial state, respectively. Notable, overexpression of and genes significantly increased the mitochondrial membrane potential (MMP) and ATP content, which were helpful to improve the antioxidant activities of the strains. On the other hand, deletion of , and caused mitochondrial damage and significantly decreased cell vitality, and caused the imbalance of intracellular ROS. The intracellular ROS content significantly increased to 174.27%, 128.68%, 200.92% of the initial state, respectively, upon HO stress for 6 h. The results showed that , and might be potential targets for regulating the antioxidant properties of yeast, providing a new clue for further research.
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