Management and housing factors associated with paratuberculosis-positive herds in small structured alpine cattle husbandry.

Paratuberculosis (Johne´s disease) is a world-wide cattle disease caused by Mycobacterium avium subsp. paratuberculosis (MAP), associated with substantial economic losses. Purchase of subclinically infected animals or contact with animals and equipment of infected farms are known risk factors for disease transmission among herds. The aim of the present study was to identify specific management factors in Austria that triggered a MAP-positive herd status and to evaluate known risk factors for the transmission in cattle in small structured alpine agricultural systems. The agriculture in the Austrian province of Tyrol is characterized by smallholder structures, including shared alpine pastures and traditional barn management techniques. The data from an extensive survey with 50 questions in 2013/2014 and the development of the MAP herd status of 5592 cattle farms by taking feces and blood samples were examined and statistically evaluated. MAP herd status was determined by combining the results of boot swab samples, manure samples, pooled and individual feces samples as well as serological antibody testing by ELISA. The statistical analysis (odds ratio; OR) showed that the use of milk replacers for calf feeding (p = 0.047, OR=0.472) and the use of straw as bedding material for cows (p = 0.032, OR=0.625) were associated with a decreased chance of being a MAP-positive herd. Further, housing cows in deep litter systems (p = 0.028, OR=2.232), the presence of slurry channels (p = 0.028, OR=1.411) and the use of solid manure in young cattle (p = 0.041, OR=1.744) were associated with an increased OR for being MAP-positive. Surprisingly, sharing of lowland pastures (p = 0.564, OR=1.080), alpine pastures (p = 0.419, OR=1.143) or farm equipment (p = 0.733, OR=0.963) and farm size (p = 0.425) had no significant influence on the MAP herd status. The identified differences compared with previously published results in respect of MAP spread in cattle might be attributed to the traditional agricultural structures, including small family-based farms and common pasture during summer in alpine regions. Results of this study contribute to the understanding of the spread of MAP in cattle farming in alpine regions.