AI Article Synopsis
- CUT&RUN (Cleavage Under Targets & Release Using Nuclease) is a technique that is gaining popularity for mapping genomic interactions between chromatin and proteins.
- It is more efficient than the traditional ChIP-seq method as it requires fewer cells, making it ideal for studies with limited samples.
- The current protocol is particularly fine-tuned for identifying histone modifications in epidermal stem cells isolated from adult mice using fluorescence-activated cell sorting (FACS).
Article Abstract
Cleavage Under Targets & Release Using Nuclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being widely used for characterizing the epigenetic landscape in many cell types as it utilizes far less cell numbers when compared to Chromatin Immunoprecipitation-sequencing (ChIP-seq), thereby making it a powerful tool for researchers working with limited material. This protocol has been specifically optimized for detecting histone modifications in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10841116 | PMC |
| http://dx.doi.org/10.1007/7651_2023_499 | DOI Listing |
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