AI Article Synopsis
- Food-borne illnesses can occur from contaminants in agricultural products, prompting the need for effective testing methods.
- The study utilized nanoplate digital PCR (dPCR), which segments the PCR mixture into over 8 million partitions for precise detection of individual DNA molecules.
- Using this method, strawberries contaminated with animal feces were tested, achieving a detection limit of 250 fg/uL, highlighting dPCR's potential for rapid and sensitive detection of contaminated produce.
Article Abstract
Food-borne illnesses can result from contamination of agricultural products. In this study, we examined nanoplate digital PCR (dPCR) to test for fecal contamination of agricultural products. In nanoplate technique, the PCR mastermix is divided into 8.526,000 partitions, providing direct detection of individual DNA molecules, with correction by Poisson distribution. In this project, strawberries were inoculated with fecal material from animals, and the result detected by nanoplate digital PCR. A detection limit of 250 fg/uL was determined. Overall, dPCR offers a quick and sensitive method to detect contaminated produce.
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| http://dx.doi.org/10.1016/j.mimet.2023.106811 | DOI Listing |
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