AI Article Synopsis
- Ly6C monocytes are a specific type of immune cell that play a key role in monitoring blood vessel health.
- NOTCH2 signaling, especially through a protein called DLL1, is crucial for the maturation of these monocytes, although the exact mechanism isn't fully understood.
- The study found that two transcription factors, BCL6 and IRF2, are important for developing Ly6C monocytes, with IRF2 being essential in certain contexts even when BCL6 is absent.
Article Abstract
Ly6C monocytes are a myeloid subset that specializes in the surveillance of vascular endothelium. Ly6C monocytes have been shown to derive from Ly6C monocytes. NOTCH2 signaling has been implicated as a trigger for Ly6C monocyte development, but the basis for this effect is unclear. Here, we examined the impact of NOTCH2 signaling of myeloid progenitors on the development of Ly6C monocytes in vitro. NOTCH2 signaling induced by delta-like ligand 1 (DLL1) efficiently induced the transition of Ly6C TREML4 monocytes into Ly6C TREML4 monocytes. We further identified two additional transcriptional requirements for development of Ly6C monocytes. Deletion of BCL6 from myeloid progenitors abrogated development of Ly6C monocytes. IRF2 was also required for Ly6C monocyte development in a cell-intrinsic manner. DLL1-induced in vitro transition into Ly6C TREML4 monocytes required IRF2 but unexpectedly could occur in the absence of NUR77 or BCL6. These results imply a transcriptional hierarchy for these factors in controlling Ly6C monocyte development.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469339 | PMC |
| http://dx.doi.org/10.1073/pnas.2220853120 | DOI Listing |
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