Tannic Acid Interfacial Modification of Prochloraz Ethyl Cellulose Nanoparticles for Enhancing the Antimicrobial Effect and Biosafety of Fungicides.

With the poorly soluble and intrinsically unstable feature, prochloraz (Pro) was confronted with lower bioavailability in the crop defense against fungal erosion. Therefore, it was a challenging project to explore the innovative antifungal compound delivery system for improving bioavailability. The superior adhesive fungicide formulation was supposed to be an efficient pathway to enhance transmembrane permeability and biological activity. According to abundant phenolic hydroxyl groups, tannic acid (TA) was an ideal modified adhesive biomaterial to improve interfacial interactions. The fundamental purpose of this research was focused on the synergistic mechanism of TA-interfacial-modified Pro-ethyl cellulose (EC) nanoparticles for improving bioavailability and biosafety. In the stability test, TA-modified Pro-EC nanoparticles had the capacity to reduce Pro initial release burst, extending a persistent validity and improving anti-photodegradation property. The toxicity index of Pro-EC and Pro-EC-TA was approximately 2.93-fold and 4.96-fold that of Pro technical against (), respectively. Compared with nonmodified EC nanoparticles, TA-modified EC nanoparticles obtained eminent transmembrane permeability and superior adherence ability to , for hydroxyl and carboxyl groups of TA to enhance interaction with target cell membranes. The contents of cellular reactive oxygen species induced by Pro-EC and Pro-EC-TA nanoparticles were about 2.31 times and 3.00 times that of the control check (CK), respectively. Compared to the CK group, the membrane potential and ergosterol values of treated with Pro-EC-TA nanoparticles were drastically reduced by 74.91 and 56.20%, respectively. In the biosafety assay, the maximum half-lethal concentration value of the TA-modified Pro-EC nanoparticles indicated that the acute toxicity of the Pro-EC-TA nanoparticles to adult zebrafish was approximately 8.34-fold reduced compared to that of the Pro technical. These findings demonstrated that the successful interfacial modification of Pro-EC nanoparticles with TA was a highly efficient, environmentally safe, and promising alternative for sustainable agricultural application, thus making the fungicide formulation process more simplified, easier fabrication, and lower cost.