AI Article Synopsis
- Nucleosomes are fundamental units of chromatin that can hinder various DNA processes, creating a need for regulatory mechanisms to manage DNA accessibility.
- The mobilization and positioning of nucleosomes are influenced by chromatin remodeling complexes and can be analyzed using the technique of micrococcal nuclease (MNase) digestion combined with chromatin immunoprecipitation (ChIP) and sequencing.
- The text provides a detailed methodology for conducting MNase digestion and MNase ChIP-seq to investigate nucleosome dynamics and interactions with chromatin-related factors.
Article Abstract
Nucleosomes are the repeating units of chromatin. The presence of nucleosomes poses a major impediment to all DNA-dependent processes. As a result, access to DNA in chromatin is dynamically regulated by many factors, including ATP-dependent chromatin remodeling complexes. Digestion of chromatin by micrococcal nuclease (MNase) followed by chromatin immunoprecipitation (ChIP) and sequencing can be leveraged to determine nucleosome occupancy, positioning, and the ability of chromatin interacting factors to alter chromatin accessibility. Here we describe the procedure for performing MNase and MNase ChIP-seq in detail.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529416 | PMC |
| http://dx.doi.org/10.1016/j.ymeth.2023.08.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!