AI Article Synopsis
- Biophysical affinity screening is a growing method in drug discovery that helps identify potential drug candidates quickly and efficiently.
- Automation plays a crucial role in high-throughput screening (HTS) by enabling the testing of many compounds against a biological target.
- The text details the automation of two specific biophysical affinity screening techniques: differential scanning fluorimetry (DSF) and the cellular thermal shift assay (CETSA), which measure changes in protein melting temperature when small molecules bind to proteins.
Article Abstract
Biophysical affinity screening is increasingly being adopted as a high-throughput hit finding technique in drug discovery. Automation is highly beneficial to high-throughput screening (HTS) since a large number of compounds need to be reproducibly tested against a biological target. Herein, we describe how we have automated two biophysical affinity screening methods that rely on a thermal shift in protein melting temperature upon small molecule binding: differential scanning fluorimetry (DSF) and the cellular thermal shift assay (CETSA).
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| http://dx.doi.org/10.1016/j.slast.2023.08.004 | DOI Listing |
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