Objectives: To develop an in-house matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) library for improved identification of species and subspecies of the Streptococcus bovis/Streptococcus equinus-complex (SBSEC).
Methods: A total of 236 SBSEC isolates from blood stream infections and culture collections, determined by whole genome sequencing to subspecies level, were grown in brain heart infusion broth. Mass spectra were collected using the Bruker MALDI Biotyper system after ethanol-formic acid extraction. Main spectral profiles from 117 isolates were used to create the "SBSEC-CMRS library." The remaining 119 spectra were used for evaluation of Bruker MALDI Biotyper (MBT) Compass Library Revision K (2022) and the SBSEC-CMRS library.
Results: The Bruker library correctly identified species and subspecies in 72 of 119 (61 %) isolates, while the SBSEC-CMRS library identified 116 of 119 (97 %), using a cutoff score of ≥2.0.
Conclusions: The SBSEC-CMRS library showed sufficient diagnostic accuracy, and can be implemented in clinical practice for SBSEC species and subspecies identification.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.diagmicrobio.2023.116045 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Partner fidelity, not geography, drives co-diversification of gut microbiota with hominids.
Biol Lett
January 2025
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA.
Bacterial strains that inhabit the gastrointestinal tracts of hominids have diversified in parallel (co-diversified) with their host species. The extent to which co-diversification has been mediated by partner fidelity between strains and hosts or by geographical distance between hosts is not clear due to a lack of strain-level data from clades of hosts with unconfounded phylogenetic relationships and geographical distributions. Here, I tested these competing hypotheses through meta-analyses of 7121 gut bacterial genomes assembled from wild-living ape species and subspecies sampled throughout their ranges in equatorial Africa.
View Article and Find Full Text PDFSochureki, the different saw- scaled viper: Challenges are many!
Med J Armed Forces India
January 2024
Resident, Department of Internal Medicine, Armed Forces Medical College, Pune, India.
Snake bite is a major cause of mortality in the Indian subcontinent. The condition is fraught with the problem of under reporting. Most bites in India are caused by the "Big 4 species," based on this, the anti-snake venom (ASV) is also sourced from these species only.
View Article and Find Full Text PDFOn the origin of an insular hybrid butterfly lineage.
Evolution
January 2025
Evolutionary Biology Program, Department of Ecology and Genetics (IEG), Uppsala University, Norbyvägen 18D, SE-752 36 Uppsala, Sweden.
A new species can form through hybridization between species. Hybrid speciation in animals has been intensely debated, partly because hard evidence for the process has been difficult to obtain. Here we report the discovery of a European hybrid butterfly lineage, a finding that can be considered surprising given the intense and long-term study of European butterflies.
View Article and Find Full Text PDFNew taxa of butterflies supported by genomic analysis.
Taxon Rep Int Lepid Surv
November 2024
Department of Biophysics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9050, USA.
Continuing with the genomic analysis of butterflies, we present a taxonomic update. As a result of this work, 3 genera, 6 subgenera, 16 species, and 2 subspecies are described as new. New genera and subgenera are (type species in parenthesis): Grishin, ( W.
View Article and Find Full Text PDFGenomic analysis reveals hidden species diversity in Fabricius (Lepidoptera: Riodinidae).
Insecta mundi
November 2024
Departments of Biophysics and Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390-9050 USA.
Genomic analysis of [Fabricius], 1807 (Lepidoptera: Riodinidae Grote, 1895) reveals species richness higher than anticipated. As a result, one subgenus, 22 species, and one subspecies are proposed as new (type species or type localities in parenthesis): Grishin, ( Prittwitz, 1865), () Grishin, (Peru: Rio Pachitea, Monte Alegre), () Grishin, (Guyana: Cuyuni-Mazaruni), () Grishin, (Brazil: Santa Catarina), () Grishin, (Panama: Darién), () Grishin, (Ecuador: Santo Domingo), () Grishin, (Peru: Cuzco), () Grishin, (Panama: Chiriquí), () Grishin, (Mexico: Tamaulipas), () Grishin, (Mexico: Nuevo León), () Grishin, (Argentina: Salta), () Grishin, (Peru: Piura), () Grishin, (Bolivia: La Paz), () Grishin, (Mexico: Sonora), () Grishin, (Peru: Madre de Dios), () Grishin, (Bolivia: La Paz), () Grishin, (Mexico: Chiapas), () Grishin, (Panama: Darién), () Grishin, (Panama: Darién), () Grishin, (Panama: Darién), () Grishin, (Panama: Panamá), () Grishin, (Peru: Cuzco), () Grishin, (Brazil: São Paulo), and () Grishin, (Brazil: Paraná). The following five taxa are (not subspecies): () Schaus, 1928, (not () (Linnaeus, 1767)), () Stichel, 1910, (not () Westwood, 1851), () Stichel, 1929, (not () Godman and Salvin, 1886), () R.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!