AI Article Synopsis
- The single-cell analysis of tissue macrophages is affected by technical issues like low and biased cell yields, fragmentation, and activation during tissue breakdown.
- These problems can result in incorrect identification of macrophage subpopulations and misrepresentation of macrophage-specific gene expression in other cell types.
- Understanding these limitations is essential for accurately studying the diverse types of tissue-resident macrophages and their biological roles.
Article Abstract
In this spotlight, we review technical issues that compromise single-cell analysis of tissue macrophages, including limited and unrepresentative yields, fragmentation and generation of remnants, and activation during tissue disaggregation. These issues may lead to a misleading definition of subpopulations of macrophages and the expression of macrophage-specific transcripts by unrelated cells. Recognition of the technical limitations of single-cell approaches is required in order to map the full spectrum of tissue-resident macrophage heterogeneity and assess its biological significance.
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| http://dx.doi.org/10.1182/blood.2023020597 | DOI Listing |
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