AI Article Synopsis
- BSN-37 is a novel antimicrobial peptide isolated from bovine spleen that shows antibacterial and immunomodulatory properties, prompting a study on its effects on mouse macrophages RAW264.7.
- The research involved using a whole gene expression microarray to identify differentially expressed lncRNAs and mRNAs following BSN-37 treatment, leading to the selection of eight lncRNAs and nine mRNAs for further validation through qRT-PCR.
- Results revealed significant changes, with 1294 lncRNAs and 260 mRNAs differing between treated and control groups, highlighting genes tied to immune responses, cell growth, and metabolism, and confirming the reliability of the data through qRT-PCR validation.
Article Abstract
Background: BSN-37, a novel antimicrobial peptide (AMP) containing 37 amino acid residues isolated from the bovine spleen, has not only antibacterial activity but also immunomodulatory activity. Recent evidence shows that long non-coding RNAs (lncRNAs) play an important role in regulating the activation and function of immune cells. The purpose of this experiment was to investigate the lncRNA and mRNA expression profile of mouse macrophages RAW264.7 stimulated by bovine antimicrobial peptide BSN-37.
Methods: The whole gene expression microarray was used to detect the differentially expressed lncRNA and mRNA between antimicrobial peptide BSN-37 activated RAW264.7 cells and normal RAW264.7 cells. KEGG pathway analysis and GO function annotation analysis of differentially expressed lncRNAs and mRNA were carried out. Eight kinds of lncRNAs and nine kinds of mRNA with large differences were selected for qRT-PCR verification, respectively.
Results: In the current study, we found that 1294 lncRNAs and 260 mRNAs were differentially expressed between antibacterial peptide BSN-37 treatment and control groups. Among them, Bcl2l12, Rab44, C1s, Cd101 and other genes were associated with immune responses and were all significantly up-regulated. Mest and Prkcz are related to cell growth, and other genes are related to glucose metabolism and lipid metabolism. In addition, some immune-related terms were also found in the GO and KEGG analyses. At the same time, real-time quantitative PCR was used to verify selected lncRNA and mRNA with differential expression. The results of qRT-PCR verification were consistent with the sequencing results, indicating that our data were reliable.
Conclusion: This study provides the lncRNA and mRNA expression profiles of RAW264.7 macrophages stimulated by antimicrobial peptide BSN-37 and helps to provide a reference value for subsequent studies on lncRNA regulation of antimicrobial peptide BSN-37 immune function.
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| http://dx.doi.org/10.2174/0929866530666230816110009 | DOI Listing |
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