Nanoplastics (NPs) and heavy metals are typical marine pollutants, affecting the gut microbiota composition and molting rate of marine organisms. Currently, there is a lack of research on the toxicological effects of combined exposure to horseshoe crabs. In this study, we investigated the effects of NPs and copper on the expression of molt-related genes and gut microbiome in juvenile tri-spine horseshoe crabs Tachypleus tridentatus by exposing them to NPs (100 nm, 10 particles L) and/or Cu (10 μgL) in seawater for 21 days. Compared with the control group, the relative mRNA expression of ecdysone receptor (EcR), retinoid x receptor (RXR), calmodulin-A-like isoform X1 (CaM X1), and heat shock 70 kDa protein (Hsp70) were significantly increased under the combined stress of NPs and Cu. There were no significant differences in the diversity and abundance indices of the gut microbial population of horseshoe crabs between the NPs and/or Cu groups and the control group. According to linear discriminant analysis, Oleobacillus was the most abundant microorganism in the NPs and Cu stress groups. These results indicate that exposure to either NPs stress alone or combined NPs and Cu stress can promote the expression levels of juvenile molting genes. NPs exposure has a greater impact on the gut microbial community structure of juvenile horseshoe crabs compared to Cu exposure. This study is helpful for predicting the growth and development of horseshoe crabs under complex environmental pollution.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.marenvres.2023.106128 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
SERIAL HEALTH ASSESSMENT WITH STANDARDIZED EXTERNAL SCORING AND HEMOLYMPH EVALUATION IN THE AMERICAN HORSESHOE CRAB ().
J Zoo Wildl Med
December 2024
National Aquarium, Baltimore, MD 21202, USA.
Serial health assessment of a managed population of American horseshoe crabs (HSC; ) was performed. Twenty HSC were examined once a month for 7 mon; this included a physical exam with a standardized scoring sheet, HR, morphometrics, and hemolymph evaluation with hemocyte count, copper concentration, and biochemistry panel. The HSC in this population had varying levels of external lesions at enrollment.
View Article and Find Full Text PDFHistopathological and histochemical analysis of the digestive tract of adult horseshoe crabs under mercury stress.
Aquat Toxicol
December 2024
South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510300, China; Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs of China, Guangzhou 510300, China; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China; Sanya Tropical Fisheries Research Institute, Sanya, Hainan 572019, China. Electronic address:
Mercury (Hg) contamination is a major environmental concern that continuously impacts marine organisms, including the mangrove horseshoe crab (Carcinoscorpius rotundicauda). As a vulnerable species, C. rotundicauda holds significant ecological and evolutionary value, making it particularly susceptible to Hg pollution and deserving of focused conservation efforts.
View Article and Find Full Text PDFPotential role of the antimicrobial peptide Tachyplesin III in regulating nontypeable Haemophilus influenzae-induced inflammation in airway epithelial cells.
Arch Microbiol
November 2024
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Article Synopsis
- Nontypeable Haemophilus influenzae (NTHi) is a major contributor to inflammation in chronic obstructive pulmonary disease (COPD), necessitating new treatments due to limitations of current drugs.
- A study investigated Tachyplesin III, an antimicrobial peptide from horseshoe crabs, and found it significantly reduced key inflammatory markers (like IL-1β and TNF-α) in human lung cells exposed to NTHi without being toxic.
- The research also showed that Tachyplesin III inhibits the NF-κB/NLRP3 signaling pathway, suggesting it could be a valuable new option for addressing NTHi-related airway inflammation in COPD patients.
A novel antimicrobial peptide CpAMP identified from Chinese horseshoe crab, Tachypleus tridentatus.
Fish Shellfish Immunol
January 2025
Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address:
Antibacterial peptide (AMP) is a crucial component of the innate immune system in most organism, play an important role in host defense processes. Many of these peptides have broad antimicrobial activity against Gram-negative, -positive bacteria and fungi. In the present study, a novel AMP named CpAMP was identified using transcriptome analysis in Chinese horseshoe crab.
View Article and Find Full Text PDFAn evolutionarily conserved function of C-reactive protein is to prevent the formation of amyloid fibrils.
Front Immunol
October 2024
Department of Biomedical Sciences, Quillen College of Medicine, East Tennessee State University, Johnson City, TN, United States.
C-reactive protein (CRP) binds to phosphocholine (PCh)-containing substances and subsequently activates the complement system to eliminate the ligand. The PCh-binding function of CRP has been conserved throughout evolution from arthropods to humans. Human CRP, in its structurally altered conformation at acidic pH, also binds to amyloid-β (Aβ) and prevents the formation of Aβ fibrils.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!