Cancer progresses silently to the terminal stage of the impossible operable condition. There are many limitations in the treatment options of cancer, but diagnosis in an early stage can improve survival rates and low recurrence. Exosomes are the biomolecules released from cancer cells and are promising candidates for clinical diagnosis. Among them, the cluster of differentiation 9 (CD9) protein is an important exosomal biomarker that can be used for exosome determination. Therefore, here, a CD9 aptamer was first synthesized and applied to an extended-gate field-effect transistor (EGFET)-type biosensor containing a disposable sensing membrane to suggest the possibility of detecting exosomes in a clinical environment. Systematically evaluating ligands using the exponential enrichment (SELEX) technique was performed to select nucleic acid sequences that can specifically target the CD9 protein. Exosomes were detected according to the electrical signal changes on a membrane, which is an extended gate using an Au microelectrode. The fabricated biosensor showed a limit of detection (LOD) of 10.64 pM for CD9 proteins, and the detection range was determined from 10 pM to 1 μM in the buffer. In the case of the clinical test, the LOD and detection ranges of exosomes in human serum samples were 6.41 × 10 exosomes/mL and 1 × 10 to 1 × 10 exosomes/mL, respectively, showing highly reliable results with low error rates. These findings suggest that the proposed aptasensor can be a powerful tool for a simple and early diagnosis of exosomes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acssensors.3c00879 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antibiotic residue detection by novel photoelectrochemical extended-gate field-effect transistor sensor.
J Hazard Mater
December 2024
College of Environmental Science and Engineering, Biomedical Multidisciplinary Innovation Research Institute, Shanghai East Hospital, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:
Residual antibiotics in the environment may pose threats to both ecological system and public health, necessitating the development of efficient analytical strategy for monitoring and control. This study proposes a photoelectrochemical extended-gate field-effect transistor (PEGFET) sensor for specific and sensitive detection of kanamycin. The sensor utilizes ITO glass as the extended gate electrode (photoelectrode) and titanium dioxide as the photosensitive material.
View Article and Find Full Text PDFA step towards non-invasive diagnosis of diabetes mellitus using synthesized MOF-MXene hybrid material with extended gate field-effect transistor integration.
J Mater Chem B
December 2024
Laboratory of Sensors, Energy and Electronic devices (Lab SEED), Department of Physics and Nanotechnology, SRMIST, Kattankulathur 603203, Tamil Nadu, India.
The increasing demand for non-invasive and non-enzymatic glucose sensors is driven by the objective of eliminating the need for blood pricks from the body and enabling enzyme-free detection of glucose for diagnosing diabetes mellitus. To address this need, we synthesized Ni MOF-MXene (Ni) hybrid material through a one-pot synthesis method, which acts as a catalyst to detect salivary glucose using an extended gate field effect transistor (EGFET) method. The resulting sensor exhibits good selectivity towards glucose over common interfering molecules such as sucrose, fructose, maltose, uric acid, and ascorbic acid under physiological conditions in saliva.
View Article and Find Full Text PDFDesign and demonstration of a temperature-resistant aptamer structure for highly sensitive mercury ion detection with BioFETs.
Talanta
February 2025
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan; College of Semiconductor Research, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:
Article Synopsis
- - This study created a temperature-resistant aptamer paired with electric double-layer Field-Effect Transistors (FETs) for detecting mercury ions effectively, leveraging the aptamer's stable hairpin structure that enhances sensitivity.
- - Two aptamer sequences with varying melting temperatures were tested, revealing that the one with a higher melting temperature demonstrated significantly improved detection sensitivity, achieving a remarkable limit of detection of 4.68 × 10 M, much lower than previous methods.
- - The research emphasized that both the thermal properties of aptamers and the surrounding temperature are crucial for optimizing sensor performance, particularly in differentiating between mercury and other heavy metals like arsenic and lead.
Fast and label-free detection of procalcitonin in human serum for sepsis using a WTe-based extended-gate field-effect transistor biosensor.
Biosens Bioelectron
January 2025
Division of Natural Science, Center for General Education, Chang Gung University, Taoyuan, 33302, Taiwan, Republic of China.
In this article, we present the first instance of depositing WTe-sensitive films with varying thicknesses (3, 4, and 5 nm) onto flexible polyimide substrates using radio-frequency sputtering. These films were used to create an extended-gate field-effect transistor (EGFET) for pH sensing and detecting procalcitonin (PCT) in the sera of patients with sepsis or bacterial infections. Among the films, the 4 nm WTe film exhibited high sensitivity (59.
View Article and Find Full Text PDFMulti-Electrode Extended Gate Field Effect Transistors Based on Laser-Induced Graphene for the Detection of Vitamin C and SARS-CoV-2.
ACS Appl Mater Interfaces
November 2024
Department of Electrical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.
Despite the clinical data showing the importance of ascorbic acid (AA or vitamin C) in managing viral respiratory infections, biosensors for their simultaneous detection are lacking. To address this need, we developed a portable and wireless device for simultaneous detection of AA and SARS-CoV-2 virus by integrating commercial transistors with printed laser-induced graphene (LIG) as the extended gate. We studied the effect of laser printing pass number and showed that with two laser printing passes (2-pass LIG), the sensor sensitivity and limit of detection (LOD) for AA improved by a factor of 1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!