Nearly a quarter of the world's population is infected with and remains long-term asymptomatic infection. Rv2626c is a latent infection-related protein regulated by DosR of . In this study, the Rv2626c protein was prokaryotically expressed and purified, and its immunobiological characteristics were analyzed using RAW264.7 cells and mice as infection models. SDS-PAGE and Western blotting analysis showed that the Rv2626c-His fusion protein was mainly expressed in soluble form and specifically reacted with the rabbit anti-H37RV polyclonal serum. In addition, we found that the Rv2626c protein bound to the surface of RAW264.7 macrophages and up-regulated the production of NO. Moreover, the Rv2626c protein significantly induced the production of pro-inflammatory cytokines IFN-γ, TNF-α, IL-6 and MCP-1, and induced strong Th1-tendency immune response. These results may help to reveal the pathogenic mechanism of and facilitate the development of new tuberculosis vaccine.
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Nearly a quarter of the world's population is infected with and remains long-term asymptomatic infection. Rv2626c is a latent infection-related protein regulated by DosR of . In this study, the Rv2626c protein was prokaryotically expressed and purified, and its immunobiological characteristics were analyzed using RAW264.
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