AI Article Synopsis
- Tooth enamel, the hardest material in the human body, is secreted by ameloblasts (AMs) but cannot regenerate once damaged due to the absence of these cells in erupted teeth.
- Researchers used single-cell RNA sequencing to analyze the developing human tooth and discovered key signaling pathways crucial for the differentiation of ameloblasts during fetal development.
- They also created a disease model for amelogenesis imperfecta using a 3D organoid system, which shows the potential for future advancements in regenerative dentistry.
Article Abstract
Tooth enamel secreted by ameloblasts (AMs) is the hardest material in the human body, acting as a shield to protect the teeth. However, the enamel is gradually damaged or partially lost in over 90% of adults and cannot be regenerated due to a lack of ameloblasts in erupted teeth. Here, we use single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) to establish a spatiotemporal single-cell census for the developing human tooth and identify regulatory mechanisms controlling the differentiation process of human ameloblasts. We identify key signaling pathways involved between the support cells and ameloblasts during fetal development and recapitulate those findings in human ameloblast in vitro differentiation from induced pluripotent stem cells (iPSCs). We furthermore develop a disease model of amelogenesis imperfecta in a three-dimensional (3D) organoid system and show AM maturation to mineralized structure in vivo. These studies pave the way for future regenerative dentistry.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629594 | PMC |
| http://dx.doi.org/10.1016/j.devcel.2023.07.013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcriptomic DN3 clock neuron subtypes regulate sleep.
Sci Adv
January 2025
Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02453, USA.
Article Synopsis
- Researchers used single-cell RNA sequencing to examine clock neurons in animal brains, particularly focusing on the DN3s, which are a major type of clock neuron in fruit flies.
- DN3s were found to be organized into 12 unique clusters with distinct gene expression patterns that set them apart from other clock neurons.
- The study revealed that certain DN3 subtypes play a significant role in promoting sleep through a specific receptor, suggesting a complex relationship between clock neurons and sleep regulation.
Spatial multiomic landscape of the human placenta at molecular resolution.
Nat Med
December 2024
Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Successful pregnancy relies directly on the placenta's complex, dynamic, gene-regulatory networks. Disruption of this vast collection of intercellular and intracellular programs leads to pregnancy complications and developmental defects. In the present study, we generated a comprehensive, spatially resolved, multimodal cell census elucidating the molecular architecture of the first trimester human placenta.
View Article and Find Full Text PDFThe Medaka approach to evolutionary social neuroscience.
Neurosci Res
October 2024
Graduate School of Life Sciences, Tohoku University, 980-8577, Japan. Electronic address:
Article Synopsis
- The review highlights advances in social neuroscience through the study of medaka fishes, focusing on their social cognition and mate choice behaviors, especially involving visual cues.
- Medaka fishes are advantageous for research due to their rich genetic resources and ease of genetic manipulation, making them ideal for studying evolutionary social neuroscience.
- The introduction of single-cell transcriptome technology, paired with 'Adaptive Circuitry Census,' promises to deepen understanding of molecular mechanisms behind visual mate preferences and mating strategies across species.
A longitudinal single-cell atlas of anti-tumour necrosis factor treatment in inflammatory bowel disease.
Nat Immunol
November 2024
Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.
Precision medicine in immune-mediated inflammatory diseases (IMIDs) requires a cellular understanding of treatment response. We describe a therapeutic atlas for Crohn's disease (CD) and ulcerative colitis (UC) following adalimumab, an anti-tumour necrosis factor (anti-TNF) treatment. We generated ~1 million single-cell transcriptomes, organised into 109 cell states, from 216 gut biopsies (41 subjects), revealing disease-specific differences.
View Article and Find Full Text PDFAn integrative single-cell atlas for exploring the cellular and temporal specificity of genes related to neurological disorders during human brain development.
Exp Mol Med
October 2024
Department of Integrated Biomedical and Life Science, Korea University, Seoul, Republic of Korea.
Single-cell technologies have enhanced comprehensive knowledge regarding the human brain by facilitating an extensive transcriptomic census across diverse brain regions. Nevertheless, understanding the cellular and temporal specificity of neurological disorders remains ambiguous due to developmental variations. To address this gap, we illustrated the dynamics of disorder risk gene expression under development by integrating multiple single-cell RNA sequencing datasets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!