AI Article Synopsis
- Improving protein stability is crucial for industrial uses, with backbone circularization being a promising technique to achieve this.
- The study aimed to find the optimal connector length for granulocyte colony-stimulating factor by systematically altering residues and testing thermal stability.
- Analysis showed that evaluating helix length, backbone angle, and hydrogen bond properties can effectively identify more stable connector lengths.
Article Abstract
Improving protein stability is important for industrial applications, and one promising method for achieving this is backbone circularization. As connector length affects stability, predicting and elucidating a more stable connector length is necessary for development of the backbone circularization method. However, the relationship between connector length and protein stability has not been completely elucidated. Here, we determined the most stable connector length for granulocyte colony-stimulating factor by changing one residue at a time to produce connector length variants and then measuring their thermal stability. Analysis of the local structures obtained from the predicted structures of the circularized variants revealed that an approach using helix length, dihedral backbone angle, and number of unbonded hydrogen bond donors and acceptors is suitable for identifying connector lengths with higher stability.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10549227 | PMC |
| http://dx.doi.org/10.1002/2211-5463.13692 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Investigation on a Novel Reinforcement Method of Grouting Sleeve Connection Considering the Absence of Reserved Reinforcing Bars in the Transition Layer.
Materials (Basel)
December 2024
Jiangsu Key Laboratory of Structure Engineering, Suzhou University of Science and Technology, Suzhou 215011, China.
In practical engineering, due to quality inspections of connections between prefabricated components and construction errors, reserved reinforcing bars in the transition layer may be partially insufficient or even completely absent. This defect significantly impacts the structural performance of sleeve connections, particularly under tensile or shear forces. This paper proposes a novel reinforcement method to address the connection issues caused by the absence of reserved reinforcing bars in the transition layer and verifies its feasibility through systematic experiments.
View Article and Find Full Text PDFMeter-Scale Long Connectorized Paper-like Polymer Waveguide Film for 100 Gbps Board-Level Optical Interconnects Application.
Polymers (Basel)
November 2024
State Key Laboratory of Advanced Optical Communication Systems and Networks, Shanghai Jiao Tong University, Shanghai 200240, China.
We design and fabricate meter-scale long connectorized paper-like flexible multimode polymer waveguide film with a large bandwidth-length product (BLP) for board-level optical interconnects application. The measured BLP of the multimode waveguide is greater than 57.3 GHz·m at a wavelength of 850 nm under the strictest overfilled launch condition with a maximum length of 2.
View Article and Find Full Text PDFThe microbiome and metatranscriptome of a panel from the mapping population reveal complex assembly and function involving host influence.
Front Plant Sci
October 2024
Department of Plant Biology, University of Georgia, Athens, GA, United States.
provide an optimal system for deciphering the host-microbiome interactions at various levels. We analyzed the pitcher microbiomes and metatranscriptomes of the parental species, and F1 and F2 generations from the mapping population ( X ) utilizing high-throughput sequencing methods. This study aimed to examine the host influences on the microbiome structure and function and to identify the key microbiome traits.
View Article and Find Full Text PDFMechanical Properties of Iron Tailing Sand Grout Sleeve Joints and Force Analysis.
Materials (Basel)
October 2024
School of Civil Engineering, Zhengzhou University, Zhengzhou 450001, China.
Article Synopsis
- This paper studies the mechanical properties and internal stress of reinforcing bar sleeve connectors using a specific grout made from ferro-tailed mineral sand.
- It examines how different grout types, ages, and reinforcement sizes affect performance in various stress conditions through experimental testing.
- Key findings reveal that smaller reinforcement diameters increase grip and bond strength, and the iron tailing sand grout performs well under large deformations and high stress, particularly in the initial anchorage section.
Influence of Connector Design on Displacement and Micromotion in Tooth-Implant Fixed Partial Dentures Using Different Lengths and Diameters: A Three-Dimensional Finite Element Study.
Materials (Basel)
September 2024
Department of Oral and Maxillofacial Prosthodontics, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Article Synopsis
- The study evaluates the effects of connector design on the displacement and micromotion of tooth-implant-supported fixed partial dentures (FPDs) when subjected to vertical loading.
- Using a mandibular model, two groups of simulated FPDs were tested: Group A with rigid connectors and Group B with non-rigid connectors.
- Results revealed that non-rigid connectors led to significantly higher displacement in various components, underscoring the crucial role of connector design in the stability and performance of dental prostheses.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!