A robust and facile colorimetric aptasensor for the detection of Salmonella Typhimurium based on the regulation of FeO@Cu@PCPy yolk-shell nanozyme activity.

Anal Chim Acta

School of Food and Bioengineering, Xihua University, Chengdu, 610000, China; Food Microbiology Key Laboratory of Sichuan Province, Chengdu, 610000, China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing, 400000, China. Electronic address:

Published: October 2023

AI Article Synopsis

  • Iron oxide nanozymes (FeO) have promise in bioanalysis and diagnosis due to their unique properties, but their low catalytic activity hinders their effectiveness.
  • Researchers developed a new type of nanozyme, FeO@Cu@poly(pyrrole-2-carboxylic acid) yolk-shell nanozymes (FeO@Cu@PCPy YSNs), which show improved catalytic activity and magnetic properties compared to traditional FeO nanozymes.
  • A colorimetric aptasensor was created using these nanozymes to detect Salmonella Typhimurium, achieving high sensitivity and successful application in real food samples.

Article Abstract

Due to their superparamagnetism and enzyme-like activity, iron oxide (FeO) nanozymes can be readily used for sample pretreatment and the generation of detection signals, and have, thus, attracted much attention in the field of bioanalysis and diagnosis. However, the low catalytic activity of FeO nanozymes does reduce the sensitivity of FeO-based methods, limiting their application. In this study, FeO@Cu@poly(pyrrole-2-carboxylic acid) yolk-shell nanozymes (FeO@Cu@PCPy YSNs) were synthesized using a facile approach and selective chemical etching technology. Compared with FeO nanozymes, the FeO@Cu@PCPy YSNs demonstrated a three-fold increase in the peroxidase-like activity, good dispersity and strong superparamagnetism. In addition, the flower-shaped structure of aptamer-complementary strand (Apt-CS) conjugates was designed on the surface of the FeO@Cu@PCPy YSNs, which effectively inhibited their peroxidase-like activity by creating a physical barrier that hindered the access of substrates to the center of the FeO@Cu@PCPy YSNs. Based on this principle, a robust and facile colorimetric aptasensor was developed for detecting Salmonella Typhimurium. The flower-shaped Apt-CS were dissociated in the presence of S. Typhimurium, promoting the recovery of FeO@Cu@PCPy YSN catalytic activity. Under optimized conditions, this proposed aptasensor successfully detected S. Typhimurium in a linear range of 3 to 3 × 10 CFU/mL, achieving a detection limit of 1 CFU/mL. Finally, the feasibility of this novel aptasensor was further validated by three actual samples, with recoveries of between 84.3% and 102%, thereby demonstrating the huge potential of the proposed aptasensor for detecting S. Typhimurium in foods.

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http://dx.doi.org/10.1016/j.aca.2023.341618DOI Listing

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A robust and facile colorimetric aptasensor for the detection of Salmonella Typhimurium based on the regulation of FeO@Cu@PCPy yolk-shell nanozyme activity.

Anal Chim Acta

October 2023

School of Food and Bioengineering, Xihua University, Chengdu, 610000, China; Food Microbiology Key Laboratory of Sichuan Province, Chengdu, 610000, China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing, 400000, China. Electronic address:

Article Synopsis
  • Iron oxide nanozymes (FeO) have promise in bioanalysis and diagnosis due to their unique properties, but their low catalytic activity hinders their effectiveness.
  • Researchers developed a new type of nanozyme, FeO@Cu@poly(pyrrole-2-carboxylic acid) yolk-shell nanozymes (FeO@Cu@PCPy YSNs), which show improved catalytic activity and magnetic properties compared to traditional FeO nanozymes.
  • A colorimetric aptasensor was created using these nanozymes to detect Salmonella Typhimurium, achieving high sensitivity and successful application in real food samples.
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