Due to their superparamagnetism and enzyme-like activity, iron oxide (FeO) nanozymes can be readily used for sample pretreatment and the generation of detection signals, and have, thus, attracted much attention in the field of bioanalysis and diagnosis. However, the low catalytic activity of FeO nanozymes does reduce the sensitivity of FeO-based methods, limiting their application. In this study, FeO@Cu@poly(pyrrole-2-carboxylic acid) yolk-shell nanozymes (FeO@Cu@PCPy YSNs) were synthesized using a facile approach and selective chemical etching technology. Compared with FeO nanozymes, the FeO@Cu@PCPy YSNs demonstrated a three-fold increase in the peroxidase-like activity, good dispersity and strong superparamagnetism. In addition, the flower-shaped structure of aptamer-complementary strand (Apt-CS) conjugates was designed on the surface of the FeO@Cu@PCPy YSNs, which effectively inhibited their peroxidase-like activity by creating a physical barrier that hindered the access of substrates to the center of the FeO@Cu@PCPy YSNs. Based on this principle, a robust and facile colorimetric aptasensor was developed for detecting Salmonella Typhimurium. The flower-shaped Apt-CS were dissociated in the presence of S. Typhimurium, promoting the recovery of FeO@Cu@PCPy YSN catalytic activity. Under optimized conditions, this proposed aptasensor successfully detected S. Typhimurium in a linear range of 3 to 3 × 10 CFU/mL, achieving a detection limit of 1 CFU/mL. Finally, the feasibility of this novel aptasensor was further validated by three actual samples, with recoveries of between 84.3% and 102%, thereby demonstrating the huge potential of the proposed aptasensor for detecting S. Typhimurium in foods.
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http://dx.doi.org/10.1016/j.aca.2023.341618 | DOI Listing |
Anal Chim Acta
October 2023
School of Food and Bioengineering, Xihua University, Chengdu, 610000, China; Food Microbiology Key Laboratory of Sichuan Province, Chengdu, 610000, China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing, 400000, China. Electronic address:
Int J Nanomedicine
July 2023
Medical Technology School of Xuzhou Medical University, Xuzhou City, Jiangsu, 221000, People's Republic of China.
Purpose: A novel CYPA-targeted, SiO encapsulated Au star@AgAu yolk-shell nanostructure (YSNS) was synthesized and used for ovarian cancer early diagnosis and therapy.
Methods: Diverse spectroscopic and microscopic methods were utilized to investigate the pattern of the yolk-shell nanostructure. In addition, in vitro and in vivo experiments were carried out.
RSC Adv
January 2023
Department of Chemistry and Chemical Engineering, Heze University Heze 274015 Shandong Province China
Yolk-shell nanoparticles (YSNs) have attracted a broad interest in the field of catalysis due to their unique structure and properties. The hollow structure of YSNs brings high porosity and specific surface areas which is conducive to the catalytic reactions. The flexible tailorability and functionality of both the cores and shells allow a rational design of the catalyst and may have synergistic effect which will improve the catalytic performance.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2020
Department of Chemistry, Fudan University, Shanghai 200438, P. R. China.
Manganese dioxide (MnO) nanostructures have aroused great interest among analytical and biological medicine researchers as a unique type of tumor microenvironment (TME)-responsive nanomaterial. However, reliable approaches for synthesizing yolk-shell nanostructures (YSNs) with mesoporous MnO shell still remain exciting challenges. Herein, a YSN (size, ∼75 nm) containing a mesoporous MnO shell and Er-doped upconversion/downconversion nanoparticle (UCNP) core with a large cavity is demonstrated for the first time.
View Article and Find Full Text PDFBiomaterials
February 2020
Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou, 510632, People's Republic of China.
Hypoxia is an important factor in forming multidrug resistance, recurrence and metastasis in solid tumors. Nanozymes respond to tumor microenvironment for tumor-specific treatment is a new and effective strategy. In this study, one-pot method was used to synthesize hollow Ru@CeO yolk shell nanozymes (Ru@CeO YSNs), which possess excellent light-to-heat conversion efficiency and catalytic performance.
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