AI Article Synopsis
- Nonspecific interactions are important for various physiological and adhesive applications but can lead to problems like surface fouling and reduced measurement accuracy.
- Common methods to reduce these nonspecific interactions include adjusting pH, using additives, and adding surfactants.
- The study introduces a new approach using Y-shape polyethylene glycol (PEG) to significantly decrease nonspecific binding, demonstrating its potential for improved data quality in scientific and biomedical fields.
Article Abstract
Nonspecific interactions play a significant role in physiological activities, surface chemical modification, and artificial adhesives. However, nonspecificity sometimes causes sticky problems, including surface fouling, decreased target specificity, and artifacts in single-molecule measurements. Adjusting the liquid pH, using protein-blocking additives, adding nonionic surfactants, or increasing the salt concentration are common methods to minimize nonspecific binding to achieve high-quality data. Here, we report that grafting heteromorphic polyethylene glycol (Y-shape PEG) with two inert terminates could noticeably decrease nonspecific binding. As a proof-of-concept, we performed single-molecule force spectroscopy and fluorescence staining imaging experiments to verify the feasibility of Y-shape PEG in blocking nonspecific interactions. Our results indicate that Y-shape PEG could serve as a prominent and efficient candidate to minimize nonspecificity for scientific and biomedical applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10419274 | PMC |
| http://dx.doi.org/10.3390/ijms241512414 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!