A chemodynamic nanoenzyme with highly efficient Fenton reaction for cancer therapy.

Chemodynamic therapy (CDT) is a rising technology for cancer therapy by converting intracellular hydrogen peroxide (HO) into hydroxyl radical (•OH) via transition-metal-containing nanoparticles (NPs) catalysis reaction (i.e. Fenton reaction) to kill tumor cells. Highly efficient Fenton reaction and favorable delivery of the catalytic NPs 'nanoenzyme' are the key for successful treatment of cancer. In this work, we developed a novel nanoenzyme MnFeO@GFP forandantitumor therapy. A new MnFeOnanoparticle containing two transition-metal-element Fe and Mn was synthesized for enhanced Fenton reaction and used to co-deliver protein with high biocompatibility through post-modification with dopamine polymerization, green fluorescent protein adsorption, and PEG coating. The enrichment of HOand glutathione (GSH) in tumor tissue provided a favorable microenvironment forgeneration of toxic free radicals. Feand GSH triggered a redox reaction to produce Fe, which in turn catalyzed HOinto •OH, with the consumption of antioxidant GSH. By combining Fewith another catalyzer, the catalytic efficiency of the nanoenzyme were greatly improved. Consequently, the nanoenzyme showed efficient antitumor ability bothand. Thus, the multifunctional CDT nanoenzyme platform shows great promising for antitumor therapy through the combination of catalyzers Feand Mnand codelivery of protein cargo.