AI Article Synopsis
- Pleural mesothelioma (PM) is an aggressive type of cancer that grows in a unique tumor microenvironment (TME), but effective cell models for studying this environment are limited.
- Researchers developed new cell models using mesothelial cells and mesothelioma-associated fibroblasts (Meso-CAFs) from patients, which included the introduction of the hTERT gene to enhance cell growth capabilities.
- The study found that while these new hTERT-transduced models maintained key characteristics of primary cells, the conditioned media from both primary and transduced Meso-CAFs significantly stimulated PM cell growth, suggesting these models are effective for studying PM interactions in its TME.
Article Abstract
Pleural mesothelioma (PM) is an aggressive malignancy that develops in a unique tumor microenvironment (TME). However, cell models for studying the TME in PM are still limited. Here, we have generated and characterized novel human telomerase reverse transcriptase (hTERT)-transduced mesothelial cell and mesothelioma-associated fibroblast (Meso-CAF) models and investigated their impact on PM cell growth. Pleural mesothelial cells and Meso-CAFs were isolated from tissue of pneumothorax and PM patients, respectively. Stable expression of hTERT was induced by retroviral transduction. Primary and hTERT-transduced cells were compared with respect to doubling times, hTERT expression and activity levels, telomere lengths, proteomes, and the impact of conditioned media (CM) on PM cell growth. All transduced derivatives exhibited elevated hTERT expression and activity, and increased mean telomere lengths. Cell morphology remained unchanged, and the proteomes were similar to the corresponding primary cells. Of note, the CM of primary and hTERT-transduced Meso-CAFs stimulated PM cell growth to the same extent, while CM derived from mesothelial cells had no stimulating effect, irrespective of hTERT expression. In conclusion, all new hTERT-transduced cell models closely resemble their primary counterparts and, hence, represent valuable tools to investigate cellular interactions within the TME of PM.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10417280 | PMC |
| http://dx.doi.org/10.3390/cells12152006 | DOI Listing |
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