cell-based bioassays have great potential for applications in the human health risk assessment of chemicals. The quantification of freely dissolved concentrations ( ) in assays is essential to generate reliable data for -to- extrapolation. Existing methods for the quantification of are limited to low-throughput microtiter plates. The present study is a proof of principle for the applicability of a solid-phase microextraction (SPME) method for the determination of in the peroxisome proliferator-activated receptor gamma (PPARγ) bioassay run in 384-well plates with 80 µL medium per well. The effect concentrations obtained from 384-well plates were compared with those obtained from 96-well plates in a previous study. Nominal effect concentrations obtained using 96- and 384-well plates agreed with each other within a factor of three, and freely dissolved effect concentrations agreed within a factor of 6.5. The good degree of agreement in the results from both plate formats proves the general applicability of the SPME method for the determination of for bioassays in 384-well plates, making the present study a first step toward exposure assessment in high-throughput bioassays.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10411540 | PMC |
| http://dx.doi.org/10.3389/ftox.2023.1221625 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Light-harvesting microelectronic devices for wireless electrosynthesis.
Nature
January 2025
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, USA.
High-throughput experimentation (HTE) has accelerated academic and industrial chemical research in reaction development and drug discovery and has been broadly applied in many domains of organic chemistry. However, application of HTE in electrosynthesis-an enabling tool for chemical synthesis-has been limited by a dearth of suitable standardized reactors. Here we report the development of microelectronic devices, which are produced using standard nanofabrication techniques, to enable wireless electrosynthesis on the microlitre scale.
View Article and Find Full Text PDFMiniaturization and characterization of patient derived hepatocellular carcinoma tumor organoid cultures for cancer drug discovery applications.
SLAS Discov
December 2024
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA; University of Pittsburgh Medical Center Hillman Cancer Center, Pittsburgh, PA 15232, USA. Electronic address:
Article Synopsis
- Patient-derived tumor organoid (PDTO) models maintain the important characteristics of original tumors, including structural and genetic diversity, making them useful for personalized medicine and drug discovery.
- The study describes how miniaturizing PDTOs into 96- and 384-well formats allows for effective growth and testing, with organoids showing increased size and number over time when cultured in specific conditions.
- High-content imaging (HCI) techniques enable in-depth analysis of organoid morphology and viability, revealing insights into drug penetration and organoid response to treatments, particularly in hepatocellular carcinoma (HCC) models.
A biosensor-based phage display selection method for automated, high-throughput Nanobody discovery.
Biosens Bioelectron
March 2025
VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Pleinlaan 2-building E, 1050, Brussels, Belgium; Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium. Electronic address:
Biopanning methods to select target-specific Nanobodies® (Nbs) involve presenting the antigen, immobilized on plastic plates or magnetic beads, to Nb libraries displayed on phage. Most routines are operator-dependent, labor-intensive and often material- and time-consuming. Here we validate an improved panning strategy that uses biosensors to present the antigen to phage-displayed Nbs in a well.
View Article and Find Full Text PDFExpedited SARS-CoV-2 Main Protease Inhibitor Discovery through Modular 'Direct-to-Biology' Screening.
Angew Chem Int Ed Engl
December 2024
Chemical Biology, GSK, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, UK.
Reactive fragment (RF) screening has emerged as an efficient method for ligand discovery across the proteome, irrespective of a target's perceived tractability. To date, however, the efficiency of subsequent optimisation campaigns has largely been low-throughput, constrained by the need for synthesis and purification of target compounds. We report an efficient platform for 'direct-to-biology' (D2B) screening of cysteine-targeting chloroacetamide RFs, wherein synthesis is performed in 384-well plates allowing direct assessment in downstream biological assays without purification.
View Article and Find Full Text PDFScreening the Global Health Priority Box against Plasmodium berghei liver stage parasites using an inexpensive luciferase detection protocol.
Malar J
November 2024
Center for Tropical and Emerging Global Diseases, University of Georgia, 500 DW Brooks Dr, Athens, GA, 30602, USA.
Article Synopsis
- - Malaria, caused by the Plasmodium parasite, faces challenges due to drug resistance, prompting the need for new treatments targeting both symptomatic and asymptomatic liver stages; a cost-effective luciferase detection method for liver stage screening has been developed using common lab reagents.
- - The study optimized a protocol for testing liver stage parasites, validating it with 28 existing anti-malarials to ensure reliable signal data, enabling the screening of the Global Health Priority Box (GHPB) for potential drug candidates.
- - Results showed that the optimized screening method produced a more stable luciferase signal with lower cell density, identifying 9 promising hits with selective activity against the P. berghei liver stages.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!