It has been first demonstrated that DNA concentration in the plasma of healthy donors was 5 to 30 mu kg per I ml of plasma or 10-50% of DNA content in leukocytes contained in the same blood volume. DNA plasma concentration was determined by registering DNA-bisbenzimide complex fluorescence in supernatant derived from plasma containing 10% NaCl upon heating for 2-3 min at 100 degrees C. The method is simple, specific and permits the determination of DNA concentrations in 0.05-0.1 ml of plasma samples.
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