In this study, direct analysis in real time high resolution mass spectrometry (DART-HRMS) was used to investigate the accurate characterisation of feed additive formulations containing coccidiostats or carotenoids. The study demonstrates the efficacy of DART-HRMS in identifying the active substances in these formulations and distinguishing between feed additives with the same active substance. The protocol for this method involves two simple steps that are extracting samples with organic solvents and measuring the extracts with DART-HRMS. The study also employs various statistical tools, including a factorial design approach, to optimise the DART-HRMS settings, and multivariate statistics, to establish classification models for feed additive formulations using nominal mass spectra. Our study demonstrates the potential of DART-HRMS in ensuring the correct identification of feed additives containing various coccidiostats or carotenoids and proposes this tool as an additional means for compliance checks with EU regulations.
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http://dx.doi.org/10.1080/19440049.2023.2240437 | DOI Listing |
J Anim Physiol Anim Nutr (Berl)
January 2025
Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, University of South Bohemia, České Budějovice, Czech Republic.
Rainbow trout (Oncorhynchus mykiss) is an important fish species raised in aquaculture, but it is susceptible to stress, infections diseases. The present study aimed to determine the effects of fulvic acid feed addition on the systemic and mucosal protective mechanisms of juvenile rainbow trout and to elucidate the underlying molecular mechanisms of changes in the gut. Rainbow trout (4.
View Article and Find Full Text PDFSci Rep
January 2025
Research Institute of Energy and Resources, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
To develop a direct production process for TiH powder from TiO, the reduction of TiO using Mg in molten MgCl - KCl salt under a high hydrogen chemical potential was investigated. The reduction of nano-sized TiO powder was conducted at 973 - 1073 K under an Ar or Ar and 10% H mixed gas atmosphere when the mass ratios of Mg to feed and salt to feed were 1.14 - 2.
View Article and Find Full Text PDFJ Nutr
January 2025
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing, China. Electronic address:
Selenium is widely acknowledged as an indispensable trace element for humans and various animals, including poultry. The addition of selenium in appropriate doses plays a crucial role in promoting poultry growth and reproduction. Conversely, both deficiency and excessive intake of selenium can pose significant threats to poultry health and production performance.
View Article and Find Full Text PDFMicrosc Microanal
January 2025
Vocational School of Health Services, Ankara Yıldırım Beyazıt University, Çubuk, Ankara 06760, Turkey.
Leeches are widely used as model organisms in scientific studies and medical treatments. Medical leeches are hematophagous parasites that usually feed on the blood of their hosts. Some leeches show deformities, usually after feeding.
View Article and Find Full Text PDFFollowing a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of NCIMB 30094 as a technological additive (functional group: silage additive) for use in fresh material for all animal species. The additive is intended for use at a proposed minimum concentration of 1 × 10 colony forming units (CFU)/kg fresh plant material. The bacterial species is considered by EFSA to be suitable for the qualified presumption of safety approach to safety assessment.
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