AI Article Synopsis
- The study aimed to create an animal model that distinguishes between joint instability and inflammation's effects on posttraumatic osteoarthritis (PTOA), proposing that their modified drilling procedure would induce cartilage damage and inflammation without altering gait.
- Twenty-four Yucatan minipigs were divided into two groups, one undergoing the modified intra-articular drilling (mIAD) and the other serving as a sham control, with outcomes assessed through various evaluations 15 weeks post-surgery.
- Results showed that mIAD knees had significantly more cartilage damage and inflammation markers than controls, but no notable changes in gait mechanics, suggesting this model is useful for studying inflammatory roles in PTOA and developing relevant therapies.
Article Abstract
Objectives: Animal models are needed to reliably separate the effects of mechanical joint instability and inflammation on posttraumatic osteoarthritis (PTOA) pathogenesis. We hypothesized that our modified intra-articular drilling (mIAD) procedure induces cartilage damage and synovial changes through increased inflammation without causing changes in gait.
Methods: Twenty-four Yucatan minipigs were randomized into the mIAD (n=12) or sham control group (n=12). mIAD animals had two osseous tunnels drilled into each of the tibia and femur adjacent to the anterior cruciate ligament (ACL) attachment sites on the left hind knee. Surgical and contralateral limbs were harvested 15 weeks post-surgery. Cartilage degeneration was evaluated macroscopically and histologically. Synovial changes were evaluated histologically. Interleukin-1 beta (IL-1β), nuclear factor kappa B (NF-κB), and tumor necrosis factor alpha (TNF-α) mRNA expression levels in the synovial membrane were measured using quantitative real-time polymerase chain reaction. IL-1β and NF-κB levels in chondrocytes were assessed using immunohistochemistry. Load asymmetry during gait was recorded by a pressure-sensing walkway system before and after surgery.
Results: The mIAD surgical knees demonstrated greater gross and histological cartilage damage than contralateral (P<.01) and sham knees (P<.05). Synovitis was present only in the mIAD surgical knee. Synovial inflammatory marker (IL-1β, NF-κB, and TNF-α) expression was three times higher in the mIAD surgical knee than the contralateral (P<.05). Chondrocyte IL-1β and NF-κB levels were highest in the mIAD surgical knee. In general, there were no significant changes in gait.
Conclusions: The mIAD model induced PTOA through inflammation without affecting gait mechanics. This large animal model has significant applications for evaluating the role of inflammation in PTOA and for developing therapies aimed at reducing inflammation following joint injury.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10408525 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Chronic intermittent hypobaric hypoxia alleviates early-stage posttraumatic osteoarthritis via NF-κB/Nrf2 pathway in mice.
J Orthop Surg Res
December 2024
Department of Orthopaedic Trauma, Hebei Medical University Third Hospital, Ziqiang Road No.139, Shijiazhuang, Hebei Province, 050051, China.
Background: Posttraumatic osteoarthritis (PTOA) is directly associated with early acute articular cartilage injury. Inhibition of cartilage destruction immediately following joint damage can effectively slow or prevent PTOA progression. Therefore, we sought to determine intervention targets and therapeutic strategies in the acute stage of cartilage injury.
View Article and Find Full Text PDFTRPC1 links calcium signaling to cellular senescence in the protection against post-traumatic osteoarthritis.
JCI Insight
December 2024
Institute of Musculoskeletal Medicine, University Hospital Münster, Münster, Germany.
Transient receptor potential channel 1 (TRPC1) is a widely expressed mechanosensitive ion channel located within the endoplasmic reticulum membrane, crucial for refilling depleted internal calcium stores during activation of calcium-dependent signaling pathways. Here, we demonstrate that TRPC1 activity is protective within cartilage homeostasis in the prevention of cellular senescence associated cartilage breakdown during mechanical and inflammatory challenge. We reveal that TRPC1 loss is associated with early stages of osteoarthritis (OA) and plays a non-redundant role in calcium signaling in chondrocytes.
View Article and Find Full Text PDFHR-pQCT measurements of changes in periarticular bone density and microarchitecture one year after acute knee injury and after reconstructive surgery.
Bone
December 2024
McCaig Institute for Bone and Joint Health, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Department of Biomedical Engineering, Schulich School of Engineering, University of Calgary, Calgary, AB, Canada; Department of Radiology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:
ACL injuries commonly lead to post-traumatic osteoarthritis (PTOA), but the underlying mechanism is not well-understood. One theorized mechanism is pathological bone remodelling following an ACL tear, for which high-resolution peripheral quantitative computed tomography (HR-pQCT) is uniquely positioned to investigate in vivo in humans. In this study, we longitudinally investigate the one-year changes in periarticular bone density and microarchitecture in the human knee following an ACL tear and reconstructive surgery using data sampled from an on-going observational cohort study.
View Article and Find Full Text PDFCSF1-R inhibition attenuates posttraumatic osteoarthritis and quadriceps atrophy following ligament injury.
J Physiol
December 2024
Center for Muscle Biology, University of Kentucky, Lexington, KY, USA.
Knee osteoarthritis contributes substantially to worldwide disability. Post-traumatic osteoarthritis (PTOA) develops secondary to joint injury, such as ligament rupture, and there is increasing evidence suggesting a key role for inflammation in the aetiology of PTOA and associated functional deficits. Colony stimulating factor 1 receptor (CSF1-R) has been implicated in the pathogenesis of musculoskeletal degeneration following anterior cruciate ligament (ACL) injury.
View Article and Find Full Text PDFTreatment of long finger metacarpophalangeal arthritis using HAPY® pyrocarbon interposition implants: a study of 34 cases.
Hand Surg Rehabil
December 2024
Institut Main Nantes Atlantique, Boulevard Charles-Gautier, 44800 Saint-Herblain, France. Electronic address:
Introduction: Destruction of the metacarpophalangeal joint can result in disabling pain and stiffness. Several therapeutic options are available, including pyrocarbon interposition implants. The primary endpoint of this study was assessment of clinical and radiographic outcomes in 34 patients treated with HAPY® pyrocarbon interposition implants (Tornier, Grenoble, France) with a minimum follow-up of 3 years.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!