Objective: Oxidative stress affects the development of ovarian follicles during culture thus applying an antioxidant is necessary. The aim of this study was to investigate the effect of coenzyme Q (CoQ) on the expression of apoptosis-related genes of mouse ovaries during culture.
Materials And Methods: The immature mouse ovaries were cultured in the presence of 50 μM CoQ for 7 days. Histological examinations were performed and the 17 beta-estradiol concentration was measured on the seventh day of culture. The relative expression of 3, , , and 2 genes were investigated by real-time RT-PCR.
Results: The rates of normal follicles in the presence of CoQ was significantly increased compared to the control group. Also, in CoQ-trated group a significant increase in the level of 17 beta-estradiol was seen compared to the control group. The mRNA expression of anti-apoptotic gene 2 was significantly increased while the expression of pro-apoptotic genes (3, and ) significantly declined in CoQ treated group compared to those of control group.
Conclusion: The supplementation of the ovarian culture media with CoQ improved the follicular development through alteration in expression of apoptosis-related genes and stimulated the production of estradiol.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10397532 | PMC |
| http://dx.doi.org/10.18502/jfrh.v17i2.12868 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Caspase 3/GSDME-Mediated Corneal Epithelial Pyroptosis Promotes Dry Eye Disease.
Invest Ophthalmol Vis Sci
January 2025
Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
Purpose: Dry eye disease (DED) is a common ocular surface inflammatory disease with a complex pathogenesis. Herein, the role and effect of gasdermin E (GSDME) in DED pathogenesis were explored.
Methods: In vitro, flow cytometry, Cell Counting Kit-8 (CCK-8) and lactate dehydrogenase (LDH) release assays were used to determine the effects of hyperosmotic stress on pyroptosis, apoptosis, and cell viability in human corneal epithelial cells (HCECs).
Strategies to Overcome Intrinsic and Acquired Resistance to Chemoradiotherapy in Head and Neck Cancer.
Cells
December 2024
Radiotherapy Department, Institut Jules Bordet, Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium.
Definitive chemoradiotherapy (CRT) is a cornerstone of treatment for locoregionally advanced head and neck cancer (HNC). Research is ongoing on how to improve the tumor response to treatment and limit normal tissue toxicity. A major limitation in that regard is the growing occurrence of intrinsic or acquired treatment resistance in advanced cases.
View Article and Find Full Text PDFTherapeutic potential of L. extract on 7,12-dimethylbenz(a)anthracene (DMBA) -induced ovary cancer in Wistar rats: a biochemical, molecular and histopathological approach.
Toxicol Res (Camb)
January 2025
Department of Obstetrics and Gynecology, Jinggangshan University Clinical School of Medicine, No. 28 Xueyuan Road, Ji'an, Jiangxi 343000, China.
Ovarian cancer (OC) is a significant cause of cancer-related mortality among women. This study explores the efficacy of L. () extract, known for its phytoestrogenic properties, in treating OC through hormonal and metabolic modulation.
View Article and Find Full Text PDFDual EGFR and telomerase inhibitory potential of new triazole tethered Schiff bases endowed with apoptosis: design, synthesis, and biological assessments.
RSC Med Chem
December 2024
Department of Pharmaceutical Chemistry, College of Pharmacy, The University of Mashreq Baghdad 10023 Iraq.
Many cancers have displayed resistance to chemotherapeutic drugs over the past few decades. EGFR has emerged as a leading target for cancer therapy inhibiting tumor angiogenesis. Besides, studies strongly suggest that blocking telomerase activity could be an effective way to control the growth of certain cancer cells.
View Article and Find Full Text PDFCarboxymethyl-β-1,3-D-glucan (CMG)-gemcitabine conjugate improves the anticancer efficacy and alleviate the toxicity of gemcitabine (GEM).
Nat Prod Res
January 2025
Institute of Functional Foods & Wines, Shenyang Pharmaceutical University, Shenyang, China.
Gemcitabine (GEM) is an antitumor drug approved by the US FDA in 1996. It is used to treat cancer and solid tumours, but its effectiveness is limited by toxicity. Carboxymethyl-β-1,3-D-glucan (CMG) is a derivative of β-glucan with improved solubility.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!