Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case-Control Approach.

Background: Recurrent miscarriage (RM), defined as two or more consecutive miscarriages prior to the 20 week of gestation is characterised by multifactorial aetiology. The prevalence of RM varies from 0.8% to 13.5% amongst women of reproductive age. The aetiological basis of RM has been traced to chromosomal, anatomic, hormonal and immunologic factors while half of the cases remain idiopathic.

Aims: This study aimed to investigate the association of interleukin-10 (IL-10) polymorphisms with RM amongst the Indian population.

Settings And Design: The present study included a total of 414 individuals including RM women ( = 199) with two or more pregnancy losses and healthy women ( = 215) without any previous history of pregnancy loss were taken as the control group.

Materials And Methods: Demographic features and reproductive history of women with RM and healthy women were taken. Genotype analysis of IL-10 polymorphisms rs1800872 and rs1800896 was performed using the polymerase chain reaction (PCR) restriction fragment length polymorphism and amplification mutation refractory system PCR, respectively.

Statistical Analysis Used: Student's -test was used to compare the demographic features and reproductive history amongst both groups. Pearson's Chi-square was used to calculate the Hardy-Weinberg equilibrium, allelic and genotypic frequencies. All the statistical analyses were performed using the SPSS (version 21, IBM SPSS, NY, USA).

Results: Our results suggested that the genotypic and allelic frequency of rs1800872 polymorphism did not differ significantly between RM cases and control women ( = 0.07 and = 0.23, respectively). The GG genotype ( = 0.007) and G allele ( = 0.003) of rs1800896 were significantly associated with an increased risk of RM. A statistically significant difference was also found for the distribution of genetic models (dominant and co-dominant model) between both groups for rs1800896. However, haplotype analysis revealed that none of the haplotypes provides a risk for the progression of RM.

Conclusion: The study is the first of its kind from our region and provides baseline data on the genetics of RM.