Objective: To explore the effects of preoperative autologous whole blood donation and autologous pure red blood cell (RBC) donation on hematopoietic stem cells (HSC), clarify the effects of transfusion by different blood components on HSC, and improve the treatment effect of autotransfusion.
Methods: Forty healthy male New Zealand rabbits were divided into five groups ( = 8) at random: control (Group A), surgery alone (Group B), surgery + blood sampling (Group C), surgery + preoperative autologous whole blood autotransfusion (Group D), and surgery + preoperative autologous pure RBC autotransfusion (Group E). The rabbits' bone marrow was collected before surgery (T1), 6 h after surgery (T2), and 24 h after surgery (T3) to detect the cell count, the reactive oxygen species (ROS) concentration, the ratio of senescent cells, and the expression of HSC-related proteins ( and ).
Results: Compared with groups A and B, the percentage of CD34+ cells in groups D and E at each time point was significantly increased, while the proportion of senescent cells, ROS, p53 and p21 were significantly decreased (P<0.05). Compared with Group C, the percentage of cells at T2 and T3 rose significantly, while the ratio of senescent cells, the ROS concentration, and the content of and declined significantly in Groups D and E ( < 0.05). Compared with Group D, the ratio of senescent cells at T2 declined significantly, the percentage of cells at T3 rose significantly, and the ROS concentration at T2 and T3 declined significantly in Group E ( < 0.05).
Conclusion: From the perspective of HSC, collection and transfusion of pure RBC is more beneficial to postoperative recovery of patients than whole blood transfusion.
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