AI Article Synopsis
- Researchers developed a method for imaging and targeting Staphylococcus aureus (S. aureus), a harmful bacteria, using specific optical probes that can "light up" the bacteria for precise detection.
- The technique involves a process where a custom substrate linked to vancomycin is cleaved by the bacteria's Sortase A enzyme, releasing a quencher and allowing for labeling that enhances visibility and tagging of the peptidoglycan.
- This approach not only enables better imaging of S. aureus but also allows for effective destruction of the bacteria using photodynamic therapy, showcasing its potential for treating various diseases associated with SrtA-positive bacteria.
Article Abstract
Imaging or killing of a specific pathogen is of significance for precise therapy. Staphylococcus aureus (S. aureus) is an infectious gram-positive bacteria relying on Sortase A (SrtA) to anchor cell surface protein on peptidoglycan. We herein report signal-on labeling of S. aureus with self-quenched optical probes featuring vancomycin-conjugated SrtA substrate that is flanked by a dabcyl moiety paired with either fluorescein or eosine photosensizer (PS). SrtA-mediated cleavage of the substrate motif releases the dabcyl quencher, leading to covalent labeling of peptidoglycan with fluorescein or PS of restored photophysical property. The dual biomarked-enabled peptidoglycan labeling enables signal-on imaging and effective photodynamic destruction of S. aureus, suggesting a protheranostic approch activatable to SrtA-positive bacteria engaged in myriad diseases.
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| http://dx.doi.org/10.1016/j.bmcl.2023.129428 | DOI Listing |
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