AI Article Synopsis
- This study examines how survivin interacts with the polycomb repressive complex 2 (PRC2), which plays a key role in silencing gene expression by modifying histone proteins.
- Researchers found that different levels of survivin in CD4 T cells correlate with varying expressions of PRC2 subunits, and studies indicated that survivin binds with PRC2 along DNA.
- Inhibiting survivin leads to increased trimethylation of histone 3 at lysine 27, suggesting that survivin may block PRC2's ability to silence genes effectively.
Article Abstract
This study investigates the role of survivin in epigenetic control of gene transcription through interaction with the polycomb repressive complex 2 (PRC2). PRC2 is responsible for silencing gene expression by trimethylating lysine 27 on histone 3. We observed differential expression of PRC2 subunits in CD4 T cells with varying levels of survivin expression, and ChIP-seq results indicated that survivin colocalizes with PRC2 along DNA. Inhibition of survivin resulted in a significant increase in H3K27 trimethylation, implying that survivin prevents PRC2 from functioning. Peptide microarray showed that survivin interacts with peptides from PRC2 subunits, and machine learning revealed that amino acid composition contains relevant information for predicting survivin interaction. NMR and BLI experiments supported the interaction of survivin with PRC2 subunit EZH2. Finally, protein-protein docking revealed that the survivin-EZH2 interaction interface overlaps with catalytic residues of EZH2, potentially inhibiting its H3K27 methylation activity. These findings suggest that survivin inhibits PRC2 function.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391610 | PMC |
| http://dx.doi.org/10.1016/j.isci.2023.106976 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Oral delivery of dihydroartemisinin for the treatment of melanoma via bovine milk exosomes.
Drug Deliv Transl Res
January 2025
Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, 221005, Uttar Pradesh, India.
Cancer, particularly skin cancer, is a major cause of mortality worldwide, with melanoma being one of the most aggressive and challenging to treat types. Current therapeutic options, such as dacarbazine (DTIC), have limitations due to dose-related toxicities like liver toxicity. Therefore, there is a need for new and effective treatments for melanoma.
View Article and Find Full Text PDFBiological Effect of Food for Special Medical Purposes (Nutramil Complex) on Melanoma Cells in In Vitro Study.
Nutrients
December 2024
Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, 30-149 Krakow, Poland.
Background/objectives: Melanoma malignum is considered the most dangerous form of skin cancer, characterized by the exceptional resistance to many conventional chemotherapies. The aim of this study was to evaluate the effect of Nutramil Complex (NC)-Food for Special Medical Purpose (FSMP), on two types of melanoma cell lines, primary WM115 and malignant WM266-4.
Methods: At 24 h after seeding, growth medium was replaced with a medium containing encoded treatments of NC or NC-CC (Nutramil Complex without calcium caseinate) at various concentrations.
Aqueous and ethanolic extracts of Moringa oleifera leaves induce selective cytotoxicity in Raji and Jurkat cell lines by activating the P21 pathway independent of P53.
Mol Biol Rep
January 2025
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Article Synopsis
- Moringa oleifera has been traditionally used in Africa and Asia for its medicinal properties and this study explores its potential anti-leukemia effects through its leaf extracts.
- The research involved treating leukemia cells with different concentrations of aqueous and ethanolic extracts and measuring cell viability, apoptosis, and gene expression.
- Results indicated that these extracts were more effective on leukemia cells compared to healthy cells, highlighting the potential for Moringa extracts to be developed as a novel treatment for leukemia.
[Evodiamine enhances the killing effect of NK cells on small cell lung cancer by regulating BIRC5].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
December 2024
Department of Oncology, Renmin Hospital of Wuhan University, Wuhan 430000, China.
Objective To investigate the effects of evodiamine (EVO) on Natural Killer (NK) cell-mediated killing in small cell lung cancer (SCLC) cells via affecting baculoviral inhibitor of apoptosis repeat containing 5 (BIRC5). Methods H446 cells and NK-92 cells were treated with EVO at different concentrations, and cell proliferation was detected using the MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay, while cell invasion was assessed using the Transwell assay. NK-92 cells and H446 cells were co-cultured at different effector-to-target ratios to detect the cytotoxicity of NK cells against H446 cells and the level of degranulation in NK-92 cells.
View Article and Find Full Text PDFA novel naphthylchalcone ([E]-4-(3-[naphthalen-2-yl]-3-oxoprop-1-en-1-yl) induces intrinsic and extrinsic apoptosis in human acute leukemia cell lines.
Fundam Clin Pharmacol
February 2025
Experimental Oncology and Hemopathies Laboratory, Clinical Analysis Department, Federal University of Santa Catarina, Florianópolis, 88040-900, Brazil.
Background: Chalcones have been described in the literature as promising antineoplastic compounds.
Objectives: Therefore, the objective of this study was to analyze the cytotoxic effect of 23 synthetic chalcones on human acute leukemia (AL) cell lines (Jurkat and K562).
Methods: Cytotoxicity assessment was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!