AI Article Synopsis
- Bovine mastitis is a major issue caused by over 150 different bacteria, making quick identification of pathogens in milk samples critical for effective treatment.
- A new Luminex beads-based multiplex assay was developed to identify six major bacteria associated with mastitis in two triplex sets.
- The assay achieved 100% specificity and a sensitivity level of 10^6 copies per reaction, similar to previous PCR methods, indicating it could serve as a rapid and cost-effective diagnostic tool for mastitis.
Article Abstract
Introduction: Bovine mastitis is caused by over 150 different microorganisms. Specific identification and quantification of multiple bacteria in a single milk sample becomes essential for rapid intervention.
Methods: In the present study a Luminex beads based multiplex assay emphasizing on the precise identification of six major bacterial pathogens of mastitis was developed. Assay was developed in two triplex sets, triplex 1 comprised of , and while triplex 2 consisted of , and .
Results: The analytical sensitivity was 10 6 copies per reaction mixture for all the six bacteria. A 100% analytical specificity was observed for simultaneous detection of these bacteria. Clinical milk samples from 100 bovine quarters were tested for validation.
Discussion: The analytical sensitivity was similar to the findings reported earlier in real time PCR multiplex assay targeting the DNA of the 11 most common bacterial species or groups in mastitis. The analytical specificity of the optimized assay was 100% similar to reported earlier for simultaneous detection of Mycoplasma spp. and for seven entric viruses of humans.The developed assay indicates a concept proof of a rapid, cost effective high throughput diagnostic tool for identification of major bacteria causing mastitis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10390833 | PMC |
| http://dx.doi.org/10.3389/fcimb.2023.1125562 | DOI Listing |
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