This study aims to evaluate and compare Surrogate Safety Measures (SSMs) at five midblock Rectangular Rapid Flashing Beacons (RRFB) and two midblock Pedestrian Hybrid Beacons (PHB) sites in Florida using extensive video data collected over the study period of July to November 2021. Computer vision and data processing resulted in four pedestrian SSMs, namely spatial gap, temporal gap, relative time to collision (RTTC) and Post Encroachment Time (PET). An initial investigation of the SSMs using Mann-Whitney-Wilcoxon tests revealed significant differences in the SSM values across different treatment types and hours of the day. Additionally, univariate regression of spatial gap, and multivariate regression of temporal gap, RTTC and PET revealed significant differences of SSMs across RRFB and PHB sites. The study considered both linear and non-linear (gamma, inverse Gaussian and lognormal) regression models. After considering various traffic and operational parameters, the data were aggregated for each pedestrian-vehicle interaction on each lane to create a total of 395 observations. The SSMs included average spatial gap, temporal gap, RTTC and PET for each interaction of pedestrian and vehicle on each lane. The results indicated that non-linear models performed better than the linear models. Moreover, the presence of the PHB, weekday, signal activation, lane count, pedestrian speed, vehicle speed, land use mix, morning period and pedestrian starting position from the sidewalk have been found to be significant determinants of the SSMs. Results also suggest temporal SSMs increase at the PHB sites compared to the RRFB sites, indicating an improvement of traffic safety at PHB sites. However, the spatial gap decreased for PHB sites compared to the RRFB sites, which suggests that pedestrians tend to start to cross the RRFB sites when they perceive vehicles to be further away than at the PHB sites.
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http://dx.doi.org/10.1016/j.aap.2023.107233 | DOI Listing |
J Hazard Mater
January 2025
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517, Japan. Electronic address:
Polyhydroxybutyrate (PHB) has attracted attention as a representative polymer for biodegradable plastics produced by microorganisms. Since information regarding the fate of PHB released into the environment is limited, it is necessary to identify them based on metagenomic information. We estimated the PHB biodegradability in coastal water samples collected from 15 near shore sites around Japan using oxygen consumption as an indicator in laboratory-scale incubation experiments and conducted 16S rRNA gene-based microbial community profiling.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Department of Chemical Engineering, National Chung Hsing University, Taichung, 402, Taiwan. Electronic address:
This study investigates the feasibility of PhaZ (PHB depolymerase derived from Caldimonas manganoxidans) in developing the PHB degradation and recycling process. PhaZ can be efficiently expressed and secreted at an OD of 0.5 and using 0.
View Article and Find Full Text PDFACS Synth Biol
December 2024
CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei 230026, China.
Bioproduction of chemicals by using engineered bacteria is promising for a circular economy but challenged the instability of the introduced plasmid by conventional methods. Here, we developed a two-plasmid INTEGRET system to reliably integrate the targeted gene into the genome, making it a powerful strain for efficient and steady bioproduction without requiring antibiotic addition. The INTEGRET system allows for gene insertion at over 75% inserting efficiency and flexibly controllable gene dosages.
View Article and Find Full Text PDFNat Commun
November 2024
Department of Biology, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Functional divergence of transcription factors (TFs) has driven cellular and organismal complexity throughout evolution, but its mechanistic drivers remain poorly understood. Here we test for new mechanisms using CORONA (CNA) and PHABULOSA (PHB), two functionally diverged paralogs in the CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-ZIPIII) family of TFs. We show that virtually all genes bound by PHB ( ~ 99%) are also bound by CNA, ruling out occupation of distinct sets of genes as a mechanism of functional divergence.
View Article and Find Full Text PDFEnviron Sci Process Impacts
June 2024
Key Lab of Marine Environmental Science and Ecology, Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, P. R. China.
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