Establishment of a proper DNA methylation landscape in mammalian oocytes is important for maternal imprinting and embryonic development. De novo DNA methylation in oocytes is mediated by the DNA methyltransferase DNMT3A, which has an ATRX-DNMT3-DNMT3L (ADD) domain that interacts with histone H3 tail unmethylated at lysine-4 (H3K4me0). The domain normally blocks the methyltransferase domain via intramolecular interaction and binding to histone H3K4me0 releases the autoinhibition. However, H3K4me0 is widespread in chromatin and the role of the ADD-histone interaction has not been studied in vivo. We herein show that amino-acid substitutions in the ADD domain of mouse DNMT3A cause dwarfism. Oocytes derived from homozygous females show mosaic loss of CG methylation and almost complete loss of non-CG methylation. Embryos derived from such oocytes die in mid-to-late gestation, with stochastic and often all-or-none-type CG-methylation loss at imprinting control regions and misexpression of the linked genes. The stochastic loss is a two-step process, with loss occurring in cleavage-stage embryos and regaining occurring after implantation. These results highlight an important role for the ADD domain in efficient, and likely processive, de novo CG methylation and pose a model for stochastic inheritance of epigenetic perturbations in germ cells to the next generation.
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http://dx.doi.org/10.1371/journal.pgen.1010855 | DOI Listing |
Plant Physiol Biochem
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Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, 3114, Bangladesh. Electronic address:
Pepper (Capsicum annuum L.) is one of the most significant vegetable crops worldwide which is known for its pungency and nutritional value. The aldehyde dehydrogenase (ALDH) superfamily encompasses enzymes critical for the detoxification of toxic aldehydes into non-toxic carboxylic acids.
View Article and Find Full Text PDFHeliyon
July 2024
SEECS, National University of Sciences and Technology (NUST), H-12, Islamabad, Pakistan.
In the aftermath of unparalleled disruptive technologies, the quantum realm has become a fundamental field of research due to unrivaled computational power and super-secure communication. In addition to conventional networks, a new word in the quantum domain is quantum network. The quantum network uses quantum communication (QC) to send quantum information bits known as qubits, to predetermined destination nodes.
View Article and Find Full Text PDFSante Ment Que
December 2024
Centre de recherche de l'Institut universitaire en santé mentale de Montréal (IUSMM), Québec, Canada.
Objectives In 2008, the National Institute of Mental Health (NIMH) published its strategic plan to structure future research aims and objectives including the development of a new method of classifying mental health disorders. This strategic plan gave rise to the Research Domain Criteria (RDoC) project, a framework aimed at establishing major functional domains of the human psyche ranging from normal to pathological. The Signature Consortium was created in 2009 to develop a data bank capable of contributing to the development of RDoC by identifying profiles, or Signatures, of a psychiatric population, collecting biological, psychosocial, and clinical indicators at critical moments in the care and follow-up of patients visiting the care structures of the Institut universitaire en santé mentale de Montréal (IUSMM).
View Article and Find Full Text PDFBJPsych Open
December 2024
Department of Psychiatry, Psychotherapy and Psychosomatics, Medical Faculty, University of Augsburg, Germany; and DZPG (German Center for Mental Health), University of Augsburg, Germany.
Background: Music therapy is a commonly used intervention added to usual care for psychiatric disorders.
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JMIR Form Res
December 2024
Center for Technology Experience, AIT - Austrian Institute of Technology, Vienna, Austria.
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