MicroRNAs (miRNAs) have been identified as crucial modulators of gene expression and to play a role in palatogenesis. The aim of this study was to explore the potential role and regulatory mechanisms of miRNAs during palatogenesis. RNA-sequencing was performed to compare the RNA expression profiles of mouse embryonic palatal shelf (MEPS) tissue between an all-trans retinoic acid (ATRA)-induced group and control group, followed by reverse transcription-quantitative polymerase chain reaction for validation, demonstrating upregulated expression of miRNA-470-5p and downregulated expression of in the ATRA-induced group. The specific binding sites of miRNA-470-5p that potentially govern expression were predicted by miRanda and TargetScan. The relationship between miRNA-470-5p and was validated in HEK293T cells by luciferase reporter assays, confirming that miR-470-5p acts directly on the 3'-untranslated region. mRNA and FGFR1 protein levels were markedly downregulated in MEPS epithelial cells over-expressing miRNA-470-5p. Functional experiments with CCK-8, cell colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) staining assays revealed that upregulated miRNA-470-5p expression could inhibit the epithelial-mesenchymal transition (EMT) of MEPS epithelial cells by targeting . These findings provide a new molecular mechanism of cleft palate formation, which can inform the development of new treatment and/or prevention targets.
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| http://dx.doi.org/10.1177/15353702231182215 | DOI Listing |
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