AI Article Synopsis
- The study evaluates how the herbal mixtures Hedyotis diffusa Willd (HDW) and Scutellaria barbata (SB) work therapeutically against clear cell renal cell carcinoma (ccRCC) by using advanced techniques like single-cell RNA sequencing (scRNA-seq) and network pharmacology.
- Researchers identified 29 crucial genes linked to the treatment effects of HDW-SB, confirming their potential in diagnosing and understanding immune responses in ccRCC through protein-protein interaction networks and molecular analysis.
- The findings highlight that specific active compounds (quercetin, luteolin, and baicalein) from HDW-SB interact with these hub genes, particularly affecting natural killer (NK) cells, and have
Article Abstract
Objective: The present study aimed to investigate the therapeutic mechanism of Hedyotis diffusa Willd (HDW) and Scutellaria barbata (SB) in ccRCC using a combination of single-cell RNA sequencing (scRNA-seq) and network pharmacology.
Methods: The active ingredients and potential molecular targets of HDW-SB were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. Gene expression data (GSE53757) were obtained from the Gene Expression Omnibus database. The hub genes of HDW-SB against ccRCC were identified via the protein-protein interaction network, and further analyzed by molecular complex detection. The roles of these genes in the diagnosis and immune infiltration of ccRCC were analyzed. The clinical significance of hub genes was verified using scRNA-seq data (GSE121638) and molecular docking.
Results: Following the PPI network analysis, 29 hub genes of HDW-SB against ccRCC were identified. All hub genes, except for , had significantly different expressions in tumor tissue and a more accurate diagnosis of ccRCC. Fifteen cell clusters were defined based on the scRNA-seq dataset, and the clusters were annotated as six cell types using marker genes. and from hub genes were highly expressed in NK cells. Three active compounds, quercetin, luteolin, and baicalein, were found to target and from the compound-target interaction network.
Conclusion: 29 hub genes of HDW-SB against ccRCC were identified and showed good performance in terms of diagnosis and prognosis. Moreover, among these hub genes docking with the main ingredients of HDW-SB, and exerted anti-ccRCC effects through NK cells.
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| http://dx.doi.org/10.2174/1386207326666230731155309 | DOI Listing |
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