Aqueous two-phase system based on pH-responsive polymeric deep eutectic solvent for efficient extraction of aromatic amino acids.

Recently, more and more attention has been paid to the construction of stimulus-responsive aqueous two-phase systems (ATPSs) for the extraction and separation of various bioactive compounds. In this work, an ATPS based on a pH-responsive polymeric deep eutectic solvent (PDES) and phosphate salt was constructed for the first time. The pH-response properties of the PDES were studied through a series of experiments. Additionally, the phase formation mechanism was studied through experiments and simulations. This novel PDES-based ATPS was used to extract aromatic amino acids (AAAs). The extraction efficiencies for tyrosine (Tyr), phenylalanine (Phe), and tryptophan (Trp) reached 95.25%, 99.05%, and 99.10%, respectively. By adjusting pH, PDES was recycled and reused. This novel and recyclable PDES-based ATPS could be an efficient method for the extraction of AAAs, which could also be applied used as a versatile and sustainable method for the extraction of other bioactive compounds in the future.