Background: Patients diagnosed with Obstructive Sleep Apnea (OSA) syndrome have a tendency towards hypoventilation, hypoxia, and hypercarbia in the perioperative period. This study hypothesized that the Oxygen Reserve Index (ORi) could predict possible hypoxia and determine difficult airways in patients at risk for OSA, as determined by the STOP-Bang questionnaire.
Methods: This prospective study included adult patients undergoing elective surgery under general anesthesia with endotracheal intubation, divided into two groups: low risk (0-2 points) and high risk (3-8 points) based on their STOP-Bang questionnaire results. The primary outcome measure was the highest ORi value reached during preoxygenation and the time to reach this value. Data were recorded at four time points: before preoxygenation (T1), end of preoxygenation (T2), end of mask ventilation (T3), and end of intubation (T4), as well as partial oxygen pressure values in T1, T2, and T4. The secondary outcome measures were the grading scale for mask ventilation, Cormack-Lehane score, tonsil dimensions, use of a stylet, and application of the burp maneuver during intubation.
Results: In the high-risk group, preoperative peripheral oxygen saturation values, the highest ORi value reached in preoxygenation, and ORi values at T3 and T4 times were lower, and the time to reach the highest ORi value was longer (p < 0.05).
Conclusion: Using ORi in patients with OSA may be useful in evaluating oxygenation, and since difficult airway is more common, ORi monitoring will better manage possible hypoxic conditions.
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http://dx.doi.org/10.1016/j.bjane.2023.07.010 | DOI Listing |
Mol Biol Rep
December 2024
Department of Medical Biochemistry, School of Medicine, Dokuz Eylul University, Izmir, 35340, Türkiye.
Background: Collagenases, a subgroup of matrix metalloproteinases (MMPs), play crucial roles in local invasion and metastasis in cancer. While substrate zymography and in situ zymography are commonly used to analyze the collagenases, traditional techniques have limitations in determining their local activities in vitro.
Objectives: We aimed to develop a new "cell in situ collagen zymography" technique to enhance the efficiency of studying local collagenase activities in vitro.
J Environ Manage
December 2024
Oceanographic Research Institute, South African Association for Marine Biological Research, 1 King Shaka Avenue, Point, PO Box 10712, Marine Parade 4056, Durban 4001 KwaZulu-Natal, South Africa; School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, KwaZulu-Natal, South Africa. Electronic address:
Sand mining is ubiquitous given the universal demand for aggregate materials for construction and other manufacturing industries. This demand is mostly met by mining fluvially derived sediment from rivers and estuaries, resulting in extensive, enduring changes to the morphological, physico-chemical and biological characteristics of aquatic ecosystems. The extent of these activities in South Africa is largely unknown due to the dynamic, ephemeral, and often clandestine operations, which are mainly located in remote or inaccessible areas.
View Article and Find Full Text PDFbioRxiv
November 2024
Department of Genetics and Genomic Sciences, Icahn School of Medicine, Mount Sinai, NY, NY, USA.
Understanding the genetic architecture of human traits is of key biological, medical and evolutionary importance. Despite much progress, little is known about how genetic architecture varies across the trait continuum and, in particular, if it differs in the tails of complex traits, where disease often occurs. Here, applying a novel approach based on polygenic scores, we reveal striking departures from polygenic architecture across 148 quantitative trait tails, consistent with distinct concentrations of high-impact rare alleles in one or both tails of most of the traits.
View Article and Find Full Text PDFJ Immunother Cancer
October 2024
Department of Investigational Cancer Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA
ACS Synth Biol
September 2024
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, P.R. China.
Genomic integration of heterologous genes is the preferred approach in industrial fermentation-related strains due to the drawbacks associated with plasmid-mediated microbial fermentation, including additional growth burden, genetic instability, and antibiotic contamination. Synthetic biology and genome editing advancements have made gene integration convenient. Integrated expression is extensively used in the field of biomanufacturing and is anticipated to become the prevailing method for expressing recombinant proteins.
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