AI Article Synopsis
- Understanding T cell function requires exploring the molecular dynamics involved in T cell activation and effector function.
- The Jurkat (JK) CD4 T cell line serves as a valuable in vitro model for studying the intracellular signaling pathways triggered by T cell receptor (TCR) activation.
- The text outlines protocols to assess TCR and costimulation-mediated activation, immunological synapse formation, and cytokine production, which are key processes in T cell responses across various pathophysiological contexts.
Article Abstract
In order to understand T cell function, it is necessary to completely decipher the molecular dynamics underlying T cell activation and effector function. In vitro easy-to-handle cellular models are valuable tools to study intracellular molecular mechanisms in live cells. The CD4 T cell line Jurkat (JK) has been widely employed to investigate intracellular signaling leading to T cell activation in response to T cell receptor (TCR) triggering. Here, we describe diverse, complementary protocols to evaluate the TCR- and costimulation-mediated T cell activation, as well as the immunological synapse assembly and cytokine production occurring as a consequence of successful early activation events. This in vitro model is extremely useful to address molecular mechanisms operating during T cell activation and effector function acting in diverse pathophysiological scenarios.
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| http://dx.doi.org/10.1016/bs.mcb.2022.09.012 | DOI Listing |
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