Cell-to-cell communication is necessary to orchestrate effective immune responses against disease-causing agents and in homeostasis. During immune synapsis, transfer of small extracellular vesicles that contain bioactive molecules, including microRNAs, occurs from the T lymphocyte to the antigen-presenting cell. In this chapter, we describe the methodology to identify and validate specific microRNAs shuttled from T lymphocytes to B cells upon immune synapse formation, and to analyze their functional impact on post-synaptic antigen-presenting cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/bs.mcb.2022.12.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Immunological synapse-driven transfer of extracellular vesicle microRNAs in primary lymphocytes.
Methods Cell Biol
July 2023
Immunology Unit from Hospital Universitario de la Princesa, Universidad Autónoma de Madrid and Instituto de investigación Sanitaria La Princesa (IIS-IP), Madrid, Spain; Intercellular Communication in the Inflammatory Response. Vascular Pathophysiology Area, National Center for Cardiovascular Research (CNIC), Madrid, Spain; Universidad Autónoma de Madrid, Madrid, Spain; Centro de Investigación Biomédica en Red, Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain. Electronic address:
Cell-to-cell communication is necessary to orchestrate effective immune responses against disease-causing agents and in homeostasis. During immune synapsis, transfer of small extracellular vesicles that contain bioactive molecules, including microRNAs, occurs from the T lymphocyte to the antigen-presenting cell. In this chapter, we describe the methodology to identify and validate specific microRNAs shuttled from T lymphocytes to B cells upon immune synapse formation, and to analyze their functional impact on post-synaptic antigen-presenting cells.
View Article and Find Full Text PDFClathrin regulates lymphocyte migration by driving actin accumulation at the cellular leading edge.
Eur J Immunol
October 2016
Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Department of Molecular & Cellular Biology, Madrid, Spain.
Lymphocyte migration, which is essential for effective immune responses, belongs to the so-called amoeboid migration. The lymphocyte migration is up to 100 times faster than between mesenchymal and epithelial cell types. Migrating lymphocytes are highly polarized in three well-defined structural and functional zones: uropod, medial zone, and leading edge (LE).
View Article and Find Full Text PDFRevisiting the putative TCR Cα dimerization model through structural analysis.
Front Immunol
February 2013
Laboratory of Immunobiology, Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School Boston, MA, USA ; Department of Pediatrics, Harvard Medical School Boston, MA, USA ; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School Boston, MA, USA.
Despite major advances in T cell receptor (TCR) biology and structure, how peptide-MHC complex (pMHC) ligands trigger αβ TCR activation remains unresolved. Two views exist. One model postulates that monomeric TCR-pMHC ligation events are sufficient while a second proposes that TCR-TCR dimerization in cis via Cα domain interaction plus pMHC binding is critical.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!